新型冠状病毒SARS-CoV-2特异性可检测靶点的确认与重叠PCR合成  

Identification and overlapping PCR synthesis of detectable SARS-COV-2 specific gene fragments

作　　者：肖正午 肖莉[2] 传军 李亚梅 彭壮 林丽美 李凯 戴爱国 庹勤慧 廖端芳 XIAO Zhengwu;XIAO Li;CHUAN Jun;LI Yamei;PENG Zhuang;LIN Limei;LI Kai;DAI Aiguo;TUO Qinhui;LIAO Duanfang(Key Laboratory for Quality Evaluation of Bulk Herbs of Hunan Province,Changsha 410208,Hunan,China;The Second Affiliated Hospital of Soochow University,Suzhou 215004,Jiangsu,China;GeneTalks Biotech Co.,Ltd.,Changsha 410010,Hunan,China;Department of Respiratory Diseases,School of Medicine,Hunan University of Chinese Medicine,Changsha 410208,Hunan,China)

机构地区：[1]湘产大宗药材品质评价湖南省重点实验室,湖南长沙410208 [2]苏州大学附属第二医院,江苏苏州215004 [3]人和未来生物科技长沙有限公司,湖南长沙410000 [4]湖南中医药大学医学院呼吸疾病研究室,湖南长沙410000

出　　处：《中南医学科学杂志》2020年第6期641-646,共6页Medical Science Journal of Central South China

基　　金：湖南省中医药防治新冠肺炎科研攻关课题(KYGG06,湘中医药〔2020〕5号).

摘　　要：高敏感的检测试剂盒的基因检测,是防控新冠疫情的关键手段之一。鉴于目前国内外新型冠状病毒(SARS-CoV-2)检测试剂盒在敏感性方面存在的不足,我们认为与其所检测靶点数量的局限性有关。本研究结合生物信息学分析和采用重叠PCR合成策略,获得了可用于新型冠状病毒检测的8个新靶点。这些靶点主要呈现为两个方面的特点:一是增加了扩增引物的SARS-CoV-2特异性;二是增强了适宜于TaqMan探针检测的SARS-CoV-2特异性。这些新的靶点有望成为未来SARS-CoV-2高灵敏度检测及试剂盒开发的重要对象。Detection kits with high sensibility are one of the key tools for COVID-19 control.The relatively low sensitivity of SARS-COV-2 detection kits presently used is partially related to the limited number of the viral gene fragments targeted.In this study,eight novel detection targets of SARS-COV-2 were obtained by combining bioinformatics analysis and overlapping PCR synthesis strategy.These targets are characterized by two aspects:SARS-COV-2 specificity of the amplified primers and being suitable for TaqMan probe detection.These new targets add important resource for high sensitivity detection and kit development of SARS-COV-2 in the future.

关 键 词：SARS-CoV-2 检测靶点 PCR 

分 类 号：R563[医药卫生—呼吸系统]