妊娠肝内胆汁淤积症胎兔肺泡液中胆汁酸水平对上皮细胞损伤的研究  

Study on the injury of epithelial cells by bile acid level in alveolar fluid of fetal rabbits with intrahepatic cholestasis of pregnancy

作　　者：刘正希 张小利 叶晓秀 LIU Zheng-xi;ZHANG Xiao-li;YE Xiao-xiu(Department of Neonatology,Affiliated Hospital of Traditional Chinese Medicine,Southwest Medical University,Luzhou 646000,China)

机构地区：[1]西南医科大学附属中医医院新生儿科,646000

出　　处：《中国实用医药》2020年第32期202-204,共3页China Practical Medicine

基　　金：泸州市人民政府-西南医科大学科技战略合作项目(项目编号:2015LZCYD-S04)。

摘　　要：目的分析妊娠肝内胆汁淤积症胎兔肺泡液中胆汁酸水平对上皮细胞损伤的情况。方法 32只孕兔,随机分为对照组和疾病组,每组16只。在成功妊娠22 d后,疾病组孕兔经颈后皮注射苯甲酸雌二醇,对照组孕兔经颈后皮下注射生理盐水,用药直至孕兔分娩。比较两组孕兔血清生化指标水平与妊娠结局;胎兔肝胆酸水平、胎心组织心肌三磷酸腺苷酸水平、胎心细胞凋亡情况、超氧化物歧化酶(SOD)及丙二醛(MDA)水平。结果疾病组孕兔天冬氨酸转氨酶、丙氨酸转氨酸、总胆汁酸、肝胆酸水平高于对照组,总胆固醇水平低于对照组,差异具有统计学意义(P<0.05);两组孕兔总胆红素水平比较差异无统计学意义(P>0.05)。疾病组孕兔的胎兔质量(32.2±2.0)g、胎盘质量(5.0±0.4)g均低于对照组的(40.5±2.5)、(5.6±0.5)g,妊娠时间(27.5±1.4)d短于对照组的(30.4±1.7)d,胎兔存活率54.4%低于对照组的100.0%,差异具有统计学意义(P<0.05)。疾病组胎兔肝胆酸、胎心组织心肌三磷酸腺苷酸水平低于对照组,胎心细胞凋亡指数高于对照组,差异均有统计学意义(P<0.05)。疾病组胎兔SOD水平明显低于对照组, MDA水平明显高于对照组,差异有统计学意义(P<0.05)。结论妊娠肝内胆汁淤积症胎兔心肌细胞有生成量障碍、氧化损伤、细胞凋亡现象,颈后皮下注射苯甲酸雌二醇可改变疾病病理表现,由此证实雌激素可诱发胆汁淤积,也是引起疾病主要因素。Objective To analyze the injury of epithelial cells by bile acid level in alveolar fluid of fetal rabbits with intrahepatic cholestasis of pregnancy. Methods A total of 32 pregnant rabbits were randomly divided into control group and disease group, with 16 rabbits in each group. After 22 d of successful pregnancy, the pregnant rabbits in the disease group received subcutaneous injection of estradiol benzoate, and the pregnant rabbits in the control group received subcutaneous injection of normal saline. The medication was administered until delivery. The serum biochemical index levels and pregnancy outcome, cholyglycine, myocardial adenosine triphosphate level in fetal heart tissue, apoptosis of fetal heart cells, superoxide dismutase(SOD) and malondialdehyde(MDA) levels were compared between the two groups. Results The levels of aspartate aminotransferase, alanine aminotransferase, total bile acid and cholyglycine of disease group were higher than those of the control group, and total cholesterol level was lower than that of the control group, and the difference was statistically significant(P<0.05). There was no statistically significant difference in total bilirubin level between the two groups(P>0.05). The fetal rabbit mass(32.2±2.0) g and the placental mass(5.0±0.4) g in the disease group were lower than(40.5±2.5) and(5.6±0.5) g in the control group, time to pregnancy(27.5±1.4) d was shorter than(30.4±1.7) d of the control group, and survival rate of fetal rabbits 54.4% was lower than 100.0% of the control group, and the difference was statistically significant(P<0.05). The cholyglycine and myocardial adenosine triphosphate level in fetal heart tissue of disease group were lower than those of the control group, and apoptosis index of fetal heart cells was higher than that of the control group, and the difference was statistically significant(P<0.05). SOD level of disease group was obviously lower than that of the control group, and MDA was obviously higher than that of the control group, and the differ

关 键 词：妊娠肝内胆汁淤积症 胎兔 肺泡液 胆汁酸 上皮细胞 

分 类 号：R714.255[医药卫生—妇产科学]