hsa-let-7b-5p靶向ΔNp63抑制HaCaT细胞的增殖、迁移和侵袭  

Hsa-let-7b-5p inhibits proliferation,migration and invasion of HaCaT cells by target-ingΔNp63

作　　者：李敏 丁毅 郭琼[1] 塔依尔·吐尔松 张美林 陈龙[4] 冯树梅[1] Li Min;Ding Yi;Guo Qiong;Tuersong Tayier;Zhang Meilin;Chen Long;Feng Shumei(School of Basic Medical Sciences,Xinjiang Medical University,2 School of Traditional Chinese Medicine,Xinjiang Medical University,3 Xinjiang Urumqi City Central Blood Station,4 Functional Center,Institute of Basic Medicine,Xinjiang Medical University,Urumqi 830011)

机构地区：[1]新疆医科大学基础医学院 [2]新疆医科大学中医学院 [3]新疆乌鲁木齐市中心血站 [4]新疆医科大学基础医学院技能中心,乌鲁木齐830011

出　　处：《中国组织化学与细胞化学杂志》2020年第4期333-338,共6页Chinese Journal of Histochemistry and Cytochemistry

基　　金：国家自然科学基金(81660324);新疆医科大学教育教学研究与改革项目(YG2019053)。

摘　　要：目的研究在人表皮细胞中hsa-let-7b-5p的作用和其对ΔNp63表达变化的影响,探讨hsa-let-7b-5p如何影响人表皮细胞的生物功能。方法人角质形成细胞株(HaCaT)分别转染hsa-let-7b-5p mimic NC、hsa-let-7b-5p mimic、hsa-let-7b-5p inhibitor和hsa-let-7b-5p inhibitor NC,通过CCK8检测细胞增殖,Transwell实验检测细胞迁移和细胞侵袭,利用miRcode网站和RNAhybrid网站预测p63和hsa-let-7b-5p结合,利用实时荧光定量PCR检测hsa-let-7b-5p及p63表达情况。结果hsa-let-7b-5p的高表达对HaCaT细胞的增殖、迁移和侵袭均有抑制作用,hsa-let-7b-5p表达的抑制则促进了HaCaT细胞的增殖、迁移和侵袭。通过网站预测发现hsa-let-7b-5p可与对皮肤表皮细胞有重要作用的p63结合;实时定量PCR分析发现hsa-let-7b-5p影响p63的表达。结论hsa-let-7b-5p可能通过靶向p63 mRNA水平而在人表皮细胞中发挥作用。Objective To study the role of microRNA hsa-let-7b-5p in human epidermal cells and its effect onΔNp63 expres-sion,in order to investigate how hsa-let-7b-5p regulates biological functions of the human epidermal cells.Methods The human kerat-inocyte line(HaCaT)was transfected with hsa-let-7b-5p mimic NC,hsa-let-7b-5p mimic,hsa-let-7b-5p inhibitor and hsa-let-7b-5p in-hibitor NC.The cell proliferation was detected by cck8 assay,cell migration and cell invasion were detected by Transwell experiments.MiRcode and RNAhybrid websites were used to predict the binding of p63 and hsa-let-7b-5p.The real-time fluorescence quantitative PCR was used to detect the expression of hsa-let-7b-5p and p63.Results The relative high expression of hsa-let-7b-5p inhibited the proliferation,migration and invasion of HaCaT cells,while the inhibition of hsa-let-7b-5p expression promoted the proliferation,mi-gration and invasion of HaCaT cells.The website predicted that hsa-let-7b-5p can bind to p63,which plays an important role in skin epidermal cells.The results of real-time quantitative PCR revealed that hsa-let-7b-5p affected the expression of p63.Conclusion hsa-let-7b-5p possibly plays a role in HaCaT cells by targeting the mRNA level of p63.

关 键 词：miRNA let-7b-5p ΔNP63 细胞增殖 细胞迁移 细胞侵袭 

分 类 号：R34[医药卫生—基础医学]