CiWRKY17基因克隆与生物信息学分析  

Cloning and Bioinformatics Analysis of CiWRKY17 Gene

作　　者：白明珠 万永青[1] 李国婧[1] 王瑞刚[1] Bai Mingzhu;Wan Yongqing;Li Guojing;Wang Ruigang(Key Laboratory of Plant Stress Physiology and Molecular Biology,Inner Mongolia Agricultural University,Hohhot,010018)

机构地区：[1]内蒙古农业大学,植物逆境生理与分子生物学自治区重点实验室,呼和浩特010018

出　　处：《基因组学与应用生物学》2020年第8期3561-3569,共9页Genomics and Applied Biology

基　　金：国家自然科学基金项目(No.31860217)资助。

摘　　要：本研究主要目的是克隆中间锦鸡儿WRKY17转录因子并对其编码蛋白的理化性质及结构特征进行生物学特性分析。首先对CiWRKY17进行基因克隆,其次将PCR扩增后得到的全长连接到克隆载体上,最后利用生物信息学工具分析CiWRKY17等蛋白的生物学特性。结果显示,CiWRKY17基因全长957 bp,编码318个氨基酸残基,此蛋白属于WRKY超家族中IId亚家族(组)的成员,是位于细胞核中的稳定亲水性蛋白,其二级结构主要由无规则卷曲、α-螺旋和β-折叠构成,其中无规则卷曲为二级结构中最主要的结构元件,并且二级结构与三级结构预测结果高度一致。最后得出结论,这8种不同植物中的WRKY17转录因子具有高度相似的结构和特征,因此应均具有与盐胁迫相关的抗性。The aim of this study was to clone the transcription factor WRKY17 from Caragana intermedia and analyze its physical and chemical properties and structural characteristics.Firstly,the gene of CiWRKY17 was cloned.Secondly,the full length of CiWRKY17 obtained by PCR was linked to the cloning vector.Finally,the biological characteristics of CiWRKY17 protein were analyzed by bioinformatics tools.The results showed that the full length of CiWRKY17 gene was 957 bp,encoding 318 amino acid residues.The protein belonged to the IId subfamily(group)of the WRKY superfamily and was a stable hydrophilic protein located in the nucleus.Its secondary structure was mainly composed of irregular curl,alpha-helix and beta-fold.Among them,irregular curl was the most important structural element in the secondary structure,and the secondary structure and tertiary structure were also found.Structural prediction results are highly consistent.Finally,it was concluded that WRKY17 transcription factors in these eight different plants had highly similar structure and characteristics,so they should all have salt stress-related resistance.

关 键 词：WRKY 基因克隆 生物学信息 

分 类 号：Q943.2[生物学—植物学] S793.3[农业科学—林木遗传育种]