不同碳源对普通小球藻和粘红酵母共培养产油脂的影响  

Effects of Different Carbon Sources on Lipid Production in Co-culture of Chlorella vulgaris and Rhodotorula glutinis

作　　者：刘方舟 任洪艳 陈红芬 邰勇 阮文权 Liu Fangzhou;Ren Hongyan;Chen Hongfen;Tai Yong;Ruan Wenquan(Jiangsu Key Laboratory of Anaerobic Biotechnology,School of Environmental and Civil Engineering,Jiangnan University,Wuxi,214122)

机构地区：[1]江南大学环境与土木工程学院,江苏省厌氧生物技术重点实验室,无锡214122

出　　处：《基因组学与应用生物学》2020年第8期3612-3619,共8页Genomics and Applied Biology

基　　金：国家自然科学基金-青年基金项目(21808087)资助。

摘　　要：微生物碳水化合物极具制备生物燃料的潜力,适宜的培养方法是很重要的。本研究以普通小球藻和粘红酵母作为培养对象,对比单独培养和共培养的生物量与油脂产量,考察不同碳源初始质量浓度(葡萄糖:2 g/L,5 g/L,7 g/L,10 g/L;甘油:1 g/L,3 g/L,5 g/L,7 g/L;乙酸钠:2 g/L,5 g/L,7 g/L,10 g/L)对菌藻共培养生长和油脂积累的影响。结果表明,3种碳源下菌藻共培养的生物量和油脂产量均高于单独培养。葡萄糖浓度7 g/L,甘油浓度5 g/L和乙酸钠浓度10 g/L为最佳碳源浓度,其中外加碳源葡萄糖可促进微生物的快速生长和油脂积累,甘油组表现出与葡萄糖组相同的碳源转化为生物量和油脂的能力,为微生物产生物柴油工业化提供适宜的碳源可能。利用气相色谱对油脂进行脂肪酸成分分析,发现3种碳源共培养获得的脂肪酸成分皆为C16~C18,符合生物柴油对碳链长度的要求。本研究通过共培养策略提高了微生物的生物量和油脂产量,优化了碳源浓度,为微生物产油脂体系提供参考。Utilization of microorganism-based carbohydrates feedstock for biofuel production has the great potential.Suitable culture methods are very important.In this study,Chlorella vulgaris(UTEX2714)and Rhodotorula glutinis were selected as the research objects.Biomass and lipid production of single culture and co-culture were compared.The effects of initial concentration of different carbon sources(Glucose:2 g/L,5 g/L,7 g/L,10 g/L;glycerol:1 g/L,3 g/L,5 g/L,7 g/L;Sodium acetate:2 g/L,5 g/L,7 g/L g/L,10 g/L)on co-culture were also investigated.The results showed that biomass and lipid production of co-culture were higher than those of the single culture under three carbon sources.The optimum concentration of carbon sources were determined:glucose7 g/L,glycerol 5 g/L and sodium acetate 10 g/L.Addition of glucose promoted growth of microorganisms and accumulated lipid rapidly.Glycerol had the same ability to convert carbon source into biomass and lipid as the glucose,which provided a suitable carbon source for the industrialization of microbial biodiesel.In addition,the fatty acids composition of lipid was analyzed by gas chromatography.It was found that the fatty acid composition ranged from C16 to C18 which met the requirements of biodiesel for carbon chain length.In this study,microbial biomass and lipid production were improved by co-culture strategy,and the carbon source concentration was optimized,which provided a reference for microbial lipid production system.

关 键 词：碳源 普通小球藻 粘红酵母 共培养 油脂 

分 类 号：TE667[石油与天然气工程—油气加工工程]