白藜芦醇诱导小鼠C2C12成肌细胞分化  

Resveratrol Induced Differentiation of C2C12 Myoblast Cells in Mice

作　　者：曹君 黄晓雷[2] 刘晓里 Cao Jun;Huang Xiaolei;Liu Xiaoli(Shenzhen Maternal and Child Health Hospital Affiliated to Southern Medical University,Shenzhen,518000;Maternal and Child Health Hospital Affiliated to Southern Medical University,Guangzhou,510405)

机构地区：[1]南方医科大学附属深圳妇幼保健院,深圳518000 [2]南方医科大学附属妇幼保健院,广州510405

出　　处：《基因组学与应用生物学》2020年第8期3757-3762,共6页Genomics and Applied Biology

摘　　要：白藜芦醇(resveratrol,Res)是研究最广泛的生物活性植物多酚之一。尽管它对内皮血管细胞、癌细胞、炎症过程和神经退行性事件的影响已有大量的文献记载,但人们对这种植物酚在分化过程中的作用,特别是在骨骼肌细胞中的作用知之甚少。本研究通过评估所细胞形状变化以及编码肌肉特异转录因子或收缩蛋白的基因的表达,探讨了白藜芦醇对小鼠骨骼肌细胞(C2C12)在非分化状态(成肌细胞)或分化状态(肌管)的影响。结果表明白藜芦醇作为促分化剂表现为细胞延长,形成肌管表型;白藜芦醇作用12 h后,肌肉促分化标志物和转录因子Scrp3开始上调,白藜芦醇作用18 h后,重链肌球蛋白含量显著增加;miRNA-133b的目标mRNA(Srf转录因子)的转录水平提升,而miRNA-133b本身受到白藜芦醇的下调。本研究结果表明白藜芦醇对骨骼肌新的促分化调节特性,至少部分是通过调节特定的miRNAs。Resveratrol(Res)is one of the most widely studied bio-active plant polyphenols.While its effect on endothelial blood vessel cells,cancer cells,inflammatory processes and neurodegenerative events is well documented,little is known about the implication of this phytophenol in differentiating processes,particularly in skeletal muscle cells.Here,we report the effects of resveratrol on mouse skeletal muscle-derived cells(C2 C12)in either a nondifferentiated(Myoblasts)or differentiated state(Myotubes)by evaluating changes in cell shape,and the expression of genes encoding muscle-specific transcription factors or contractile proteins.Resveratrol does not strongly affect cell viability,cell cycle and apoptosis,and behaves as a pro-differentiating agent as shown by the lengthening of cells,leading to a myotube phenotype.Resveratrol upregulates muscular pro-differentiation markers and transcription factors Scrp3 starting after 12 h of exposure and strongly increases heavy chain myosin content after 18 h of exposure to resveratrol.Besides,resveratrol increases the Srf transcription factor’s transcript level,a target mRNA of the miRNA-133 b,which is itself downregulated by this polyphenol.These results put forward new pro-differentiating regulatory properties of resveratrol on skeletal muscles at least partly via modulation of specific miRNAs.

关 键 词：白藜芦醇 C2C12成肌细胞 分化 MICRORNAS 

分 类 号：R285.5[医药卫生—中药学]