低氧中强度训练改善肥胖者的身体成分和血脂代谢的分子机制研究  

Study on the Molecular Mechanism of Hypoxic and Medium-intensity Training to Improve Body Composition and Lipid Metabolism in Obese

作　　者：燕鹏 Yan Peng(Physical Education Department of Shanxi University of Traditional Chinese Medicine,Taiyuan,030024)

机构地区：[1]山西中医药大学体育教学部,太原030024

出　　处：《基因组学与应用生物学》2020年第8期3777-3785,共9页Genomics and Applied Biology

摘　　要：为了考察低氧中强度训练改善肥胖者的身体成分和血脂代谢的分子机制,本研究选择60名高校无系统运动训练史的健康男性作为研究对象,将受试者分为低氧组和对照组,每组30名。低氧组在氧浓度约为15%的训练室内进行中强度训练(65%V02max),对照组在常氧条件下进行中强度训练,共训练4周。另外,将80只6周龄雄性SD大鼠随机分为对照组、肥胖组(高脂饮食饲养)、常氧组(高脂饮食饲养+常氧跑台运动)和低氧组(高脂饮食饲养+低氧跑台运动),低氧条件与受试者相同,跑台坡度为5°,速度为16 m/min,时间为1 h,共训练4周,分别检测受试者运动前后的身体成分(体重,脂肪重量,BMI)和血脂代谢指标(血清TC,TG,HDL-C和LDL-C)。此外应用全自动生化分析仪检测各组大鼠血清AST、ALT和LDH水平,应用试剂盒检测血清SOD、GSH-PX和MDA水平。通过苏木精-伊红(HE)染色检查大鼠肝脏病理变化。通过免疫组织化学检测肝组织NF-κB、TNF-α、COX-2、P53和Bcl-2的阳性表达。研究显示,与常氧组比较,低氧组受试者运动后的体重、脂肪重量、BMI、TC、TG和LDL-C水平均显著降低,而HDL-C显著升高(p<0.05)。与常氧组比较,低氧组大鼠的AST、ALT和LDH水平均显著降低,并且肝组织病理损伤明显减轻(p<0.05)。与常氧组比较,低氧组的SOD和GSH-PX水平均显著升高,而MDA显著降低(p<0.05)。与常氧组比较,低氧组的NF-κB、TNF-α、COX-2和P53阳性染色评分均显著降低,而Bcl-2显著升高(p<0.05)。本研究表明,低氧中强度训练可显著降低肥胖者身体成本并改善血脂代谢。低氧中强度训练可通过纠正机体氧化-抗氧化失衡、减弱炎症反应、抑制细胞凋亡来减轻肥胖引起的肝组织损伤。In order to investigate the molecular mechanism of hypoxic and medium-intensity training to improve body composition and lipid metabolism in obese people,60 healthy men with no history of systemic exercise training were selected as research objects in this study.The subjects were divided into hypoxic groups and control groups,30 people in each group.The hypoxic group performed medium-intensity training(65%V02 max)in a training room with an oxygen concentration of about 15%,and the control group performed medium-intensity training under normoxic conditions for a total of 4 weeks.In addition,806-week-old male SD rats were randomly divided into a control group,an obese group(high-fat diet feeding),a normoxic group(high-fat diet feeding+normoxic treadmill exercise),and a hypoxia group(high-fat diet feeding+hypoxic treadmill exercise),the hypoxic conditions were the same as the subjects,the gradient of the treadmill was 5 degrees,the speed was 16 m/min,and the time was 1 h,trained for 4 weeks.The body composition(body weight,fat weight,BMI)and blood lipid metabolism indexes(serum TC,TG,HDL-C and LDL-C)of the subjects before and after exercise were measured.In addition,the serum AST,ALT and LDH levels of rats in each group were measured by a fully automatic biochemical analyzer,and the serum SOD,GSH-PX and MDA levels were measured by a kit.Hematoxylin-eosin(HE)staining was used to examine liver pathological changes in rats.The positive expressions of NF-κB,TNF-α,COX-2,P53 and Bcl-2 werr detected by immunohistochemistry.The study showed that compared with the normoxic group,the body weight,fat weight,BMI,TC,TG,and LDL-C levels of the subjects in the hypoxia group were significantly reduced,while HDL-C was significantly increased(p<0.05).Compared with the normoxic group,the levels of AST,ALT and LDH in the hypoxic group were significantly reduced,and the pathological damage of the liver tissue was significantly reduced(p<0.05).Compared with the normoxic group,the levels of SOD and GSH-PX in the hypoxic group increased

关 键 词：低氧中强度训练 身体成分 血脂代谢 氧化-抗氧化 

分 类 号：G804.2[文化科学—运动人体科学]