携HMME-Gd液态氟碳纳米粒造影剂的制备及体外双模态成像研究  

Preparation of HMME-Gd liquid fluorocarbon nanoparticle contrast agent and study on dual-mode imaging in vitro

作　　者：罗远利 乔斌 曹进[1] 袁勋 谢卓晏 杨安宇[1] 胡兴华 周志益 王志刚 任建丽[1] LUO Yuanli;QIAO Bin;CAO Jin;YUAN Xun;XIE Zhuoyan;YANG Anyu;HU Xinghua;ZHOU Zhiyi;WANG Zhigang;REN Jianli(Department of Ultrasound,the Second Affiliated Hospital of Chongqing Medical University,Chongqing 400010,China)

机构地区：[1]重庆医科大学附属第二医院超声科,重庆市400010 [2]重庆医科大学附属第二医院眼科,重庆市400010 [3]重庆医科大学附属第二医院神经外科,重庆市400010 [4]中国科学院大学重庆医院重庆市人民医院全科医学科 [5]重庆市超声影像学研究所

出　　处：《临床超声医学杂志》2020年第11期801-805,共5页Journal of Clinical Ultrasound in Medicine

基　　金：国家自然科学基金项目(81873901);重庆市自然科学基金项目(cstc2019jcyj-zdxmX0020);宽仁骨干人才项目(KR2019G001);重庆市渝中区基础研究与前沿探索项目(20190112)。

摘　　要：目的制备携血卟啉单甲醚-钆(HMME-Gd)的液态氟碳纳米粒(HMME-Gd-PFPNPs),观察其体外超声/MRI的成像效果。方法以磷脂、胆固醇、HMME-Gd及全氟戊烷(PFP)为原料,采用薄膜水化法和乳化法制备HMME-Gd-PFPNPs。倒置光学显微镜和透射电子显微镜下观察其基本表征;粒径分析仪检测其粒径和表面电位;紫外分光光度计分析HMME-Gd的包封率;观察HMME-Gd-PFPNPs体外超声/MRI成像的效果。结果成功制备HMMEGd-PFPNPs,光镜和电镜下均表现为形态规则、大小均一的球形。HMME-Gd-PFPNPs平均粒径为(250.27±11.9)nm,平均电位为(-26.17±0.45)mV,HMME-Gd的包封率为(84.70±0.35)%。在低强度聚焦超声辐照下,随着激发强度增加,体外超声成像信号显著增强。同时纳米粒的体外MRI成像效果具有HMME-Gd-PFPNPs浓度依赖性。结论成功制备了HMME-Gd-PFPNPs,其可用于体外超声/MRI双模态成像。ObjectiveTo prepare liquid fluorocarbon nanoparticles carrying HMME-Gd(HMME-Gd-PFPNPs),and to observe their ultrasound(US)/MRI imaging in vitro.MethodsLipids,cholesterol,HMME-Gd and perfluoropentane(PFP)were used as raw materials to prepare nanoparticles by membrane hydration and emulsification.The general physical properties were observed by optical microscope and transmission electron microscope.The size and surface potential were measured by particle size analyzer.The encapsulation efficiency of HMME-Gd was determined by UV-Vis.The effect of US/MRI in vitro of HMME-Gd-PFPNPs was observed.Results HMME-Gd-PFPNPs were successfully prepared.Under optical microscope and transmission electron microscope,it was spherical with regular shape and uniform size.The size of HMME-Gd-PFPNPs was(250.27±11.9)nm,the surface potential was(-26.17±0.45)m V,and the encapsulation efficiency of HMME-Gd was(84.70±0.35)%.Under low intensity focused ultrasound(LIFU)irradiation,the US imaging signal was significantly enhanced in vitro with the intensity of LIFU increased.At the same time,the MRI effect of nanoparticles in vitro was concentration dependent on HMME-Gd-PFPNPs.ConclusionHMME-Gd-PFPNPs is successfully prepared,and it can be used for US/MRI dual-model imaging in vitro.

关 键 词：纳米粒 造影剂 超声成像 磁共振成像 

分 类 号：R445.1[医药卫生—影像医学与核医学] TB383[医药卫生—诊断学]