基于DNA条形码技术的舟山海域石莼附生动物的分子生物学鉴定  

Species identification of epizoans from Ulva lactuca in Zhoushan sea area by using the DNA barcode technology

作　　者：刘金林 夏菁 赵莉娟 谷凯 康新宇 丁晓玮 赵晓惠 庄旻敏 张建恒 何培民 LIU Jin-lin;XIA Jing;ZHAO Li-juan;GU Kai;KANG Xin-yu;DING Xiao-wei;ZHAO Xiao-hui;ZHUANG Min-min;ZHANG Jian-heng;HE Pei-min(College of Marine Ecology and Environment,Shanghai Ocean University,Shanghai 201306,China;North China Sea Environmental Monitoring Center,State Oceanic Administration,Qingdao 266033,China;State Key Lab of Estuarine&Coastal Research,East China Normal University,Shanghai 200062,China)

机构地区：[1]上海海洋大学海洋生态与环境学院,上海201306 [2]国家海洋局北海环境监测中心,山东青岛266033 [3]华东师范大学河口海岸学国家重点实验室,上海201306

出　　处：《海洋环境科学》2020年第6期874-879,886,共7页Marine Environmental Science

基　　金：国家重点研发计划“蓝色粮仓科技创新”专项(2018YFD0901500);国家自然科学基金(41576163);国家海洋局公益性科研专项(201205010);上海市青年科技英才扬帆计划(17YF1407900);骆肇荛科创基金(A1200400201307,A1200400201328)。

摘　　要：我国绿潮暴发已持续10余年,对生态环境、旅游业等造成了严重影响。本文于2018年10月在舟山市枸杞岛附近海域采集石莼附生动物,并使用DNA条形码技术对其开展物种鉴定,旨在给绿潮暴发的生态防治提供数据参考。鉴定结果显示:附生动物主要分为麦秆虫亚目以及钩虾亚目,部分样品无法鉴定出具体种类,存在出现新种的可能性。优势种包括Caprella scauroides和Ampithoe lacertosa等,其中Ampithoe lacertosa在中国近海为首次记录。在上述基础之上,我们进一步探讨了各季节钩虾亚目不同物种对于藻类的取食偏好性,阐明了端足类生物在维持生态系统稳定性中发挥的重要作用,为防控绿潮暴发提供了新的思路。Green algal blooms have occurred along the coast of China for more than 10 years, resulting in tremendous economic loss due to the destruction of marine ecosystems and damage to ecological service functions. This research was conducted to explore the biodiversity of epizoans attached on the green tide algae,Ulva lactuca. In October 2018, Ulva lactuca was collected from the sea area near Gouqi island, Zhoushan, and DNA barcode technology was used to carry out species identification. The results showed that the epizoans were mainly divided into the Caprellidea and Gammaridea. Some samples could not be identified, so there was the possibility of the emergence of new species. The dominant species included Caprella scauroides and Ampithoe lacertosa, etc, and Ampithoe lacertosa was recorded in the offshore of China for the first time. On the basis of the above, we further discussed the important role played by amphipods in maintaining the stability of the ecosystem, and clarified that it will be of great application value to study the feeding preference of different Gammaridea species in different seasons, which provides a new idea for the prevention and control of green tide outbreaks.

关 键 词：绿潮 石莼 附生动物 Cox 1 DNA条形码 

分 类 号：Q178.53[生物学—水生生物学]