‘西伯利亚'百合类钙调蛋白基因LiCML的克隆与表达特征分析  

Cloning and Expression Analysis of Calmodulin Gene LiCML from Lilium

作　　者：李迎迎 闫子飞 马波 王浩楠 胡增辉[1,2] 冷平生[1,2] Li Yingying;Yan Zifei;Ma Bo;Wang Haonan;Hu Zenghui;Leng Pingsheng(School of Landscape Architecture,Beijing University of Agriculture,Bejing,102206;Beijing Advanced Innovation Center for Tree Breeding by Molcelar Design,Beijing Univensity of Agriulture,Beijing,102206)

机构地区：[1]北京农学院园林学院,北京102206 [2]北京农学院,林木分子设计育种高精尖创新中心,北京102206

出　　处：《分子植物育种》2020年第22期7334-7341,共8页Molecular Plant Breeding

基　　金：国家自然科学基金(31201645);北京市自然科学基金(6172006);北京市属高等学校创新团队建设与教师职业发展计划项目(IDHT20180509);北京市教委-自然科学基金委联合科技计划重点项目(KZ201800200)共同资助

摘　　要：类钙调素是一种重要的钙感受器,广泛存在于真核植物中,参与各种生理活动的信号转导。为探明其在‘西伯利亚’百合(Lilium‘Siberia’)中的作用,本研究以‘西伯利亚’百合(Lilium‘Siberia’)为材料,通过RACE技术和RT-PCR技术克隆得到了百合类钙调蛋白基因,将其命名为LiCML,并进行了时空表达分析。序列分析显示,该基因全长713 bp,开放阅读框为591 bp,编码196个氨基酸,分子量与等电点分别为22.07 kD和4.73,属于EFh超基因家族。亚细胞定位显示LiCML基因主要定位于细胞质中。qRT-PCR分析结果表明,LiCML基因在‘西伯利亚’百合四个花期中,花蕾期的表达量最高;在盛花期的九个不同器官中,LiCML基因在花药中表达量最高,与在其他不同器官中的表达量差异显著。本研究结果为LiCML基因在百合中的表达模式的研究提供了参考依据,为今后研究该基因的功能以及在百合花香释放中的信号作用提供帮助。Calmodulin is an important calcium receptor, which widely exists in eukaryotic plants and participates in signal transduction of various physiological activities. In order to find out its role in ’Siberian’ lily(Lilium’Siberia’), the calmodulin gene of Lilium sibirica(Lilium ’Siberia’) was cloned by RACE and RT-PCR, named Li CML, and the temporal and spatial expression was analyzed. Sequence analysis showed that the gene was 713 bp in length, 591 bp in open reading frame, encoding 196 amino acids, 22.07 kD in molecular weight and 4.73 in isoelectric point, belonging to the EFh supergene family. Subcellular localization showed that the LiCML gene was mainly located in cytoplasm. The results of qRT-PCR analysis showed that the LiCML gene had the highest expression in the flower bud stage in the four flowering stages of ’Siberian’ lily. Among the nine different flower organs in the full flowering stage, LiCML gene had the highest expression in anther, which was significantly different from that in other organs. The results of this study provide reference for the study of the expression pattern of Li CML gene in lily, which can be helpful for the future study of the function of this gene and the signalling role in the release of lily flower.

关 键 词：‘西伯利亚’百合 基因克隆 LiCML 基因表达 

分 类 号：S682.29[农业科学—观赏园艺] Q943.2[农业科学—园艺学]