胀果甘草中NAC转录因子基因鉴定及盐胁迫下差异表达分析  

Identification of NAC Transcription Factors and Differentially Expressed Genes Analysis under Salt Stress in Glycyrrhiza inflata

作　　者：刘珊 梁玉玲[1] Liu Shan;Liang Yuling(College of Life Science,Hebei University,Baoding,071002)

机构地区：[1]河北大学生命科学学院,保定071002

出　　处：《分子植物育种》2020年第22期7385-7394,共10页Molecular Plant Breeding

基　　金：河北省生物学一流学科建设项目、河北大学自然科学研究计划项目(No.799207217054);部省合建优势特色学科——生物学(050001-521000103001)共同资助。

摘　　要：NAC转录因子广泛作用于植物生长发育的各个阶段,具有应对逆境胁迫的作用。本研究利用生物信息学方法从胀果甘草愈伤组织转录组中鉴定出86个胀果甘草NAC转录因子基因和14个NAC差异表达基因,将14个差异表达基因分别命名为GiNAC01~GiNAC14。系统进化树聚类分析结果表明,胀果甘草愈伤组织转录组中的NAC家族基因被分为13个小组,14种NAC差异表达基因主要聚集在第5小组和第13小组。胀果甘草NAC差异表达基因与拟南芥NAC家族基因推定蛋白质构建的进化树被分为11个组,其中差异表达基因主要聚集在第1小组和第4小组。序列分析结果表明胀果甘草NAC差异表达基因推定氨基酸组成中富含丝氨酸和酸性氨基酸、脂肪族氨基酸占比较大;蛋白质二级结构主要组成元件是无规则卷曲,亚细胞定位多数定位于细胞核;蛋白质大多无跨膜螺旋和二硫桥结构,无信号肽结构,多为亲水性蛋白质。本研究丰富和完善了胀果甘草NAC基因的探索,为进一步研究胀果甘草NAC基因响应逆境胁迫的分子机制及功能奠定了基础。NAC transcription factors act extensively on all stages of plant growth and development and have a role in coping with stress. In this research, 86 Glycyrrhiza inflata NAC transcription factor genes and 14 NAC differentially expressed genes were identified from the transcriptome of Glycyrrhiza inflata callus by using bioinformatics methods. The 14 differentially expressed genes were named GiNAC01~GiNAC14. Phylogenetic tree cluster analysis showed that the NAC family genes in the transcriptome of the licorice callus were divided into 13 groups,and 14 NAC differentially expressed genes were mainly clustered in the 5 th and 13 th groups. The phylogenetic tree of the NAC differentially expressed gene and the Arabidopsis NAC family gene protein were divided into 11 groups, and the differentially expressed genes were mainly concentrated in the 1 st group and the 4 th group. The results of sequence analysis indicated that the differentially expressed genes of NAC were significantly rich in serine and acidic amino acids, and the fatty amino acids accounted for a large proportion. Most of the proteins have no transmembrane helix and disulfide bridge structure, no signal peptide structure, and most of them are hydrophilic proteins. This study enriched and improved the exploration of NAC gene of licorice, and laid a foundation for further study on the molecular mechanism and function of NAC gene in response to stress.

关 键 词：胀果甘草(Glycyrrhiza inflate) 盐胁迫 NAC基因 生物信息学 表达模式 

分 类 号：S567.71[农业科学—中草药栽培] Q943.2[农业科学—作物学]