悬钩子属(Rubus L.)种质资源DNA指纹图谱构建及遗传多样性分析  

Construction of DNA Fingerprinting and Analysis of Genetic Diversity for Rub us Germplasm Resources

作　　者：王珊珊 耿佳麒 赵晨辉[1] 宋宏伟[1] 梁英海[1] 李红莲[1] 唐雪东[3] 张冰冰[1] Wang Shanshan;Geng Jiaqi;Zhao Chenhui;Song Hongwei;Liang Yinghai;Li Honglian;Tang Xuedong;Zhang Bingbing(Pomology Institute,Jilin Academy of Agnhculture Sciences,Gongzbuling,130033;College of Landscape Architecture,Changchun University,Changchun,130022;College ofHoriculture,Jilin Agricultural University,Changchun,130118)

机构地区：[1]吉林省农业科学院果树研究所,公主岭130033 [2]长春大学园林学院,长春130022 [3]吉林农业大学园艺学院,长春130118

出　　处：《分子植物育种》2020年第22期7600-7612,共13页Molecular Plant Breeding

基　　金：寒地果树种质资源收集、鉴定编目繁殖更新与保存分发利用项目(111821301354052004);国家寒地果树种质资源子平台项目(NICGR2018-59)共同资助

摘　　要：本研究利用SSR分子标记技术,经毛细管电泳法检测PCR产物,构建了94份悬钩子属(Rubus. L)种质资源DNA指纹图谱,并对其遗传多样性进行分析。结果表明,从40对引物中筛选出13对SSR引物,其多态性高,稳定性好,共扩增出242个等位基因,多态性位点为242个,多态性比率达100%,检测到的位点数在11~27之间,平均为18.615 4;多态性信息含量(PIC)值变幅在0.635 3~0.915 1之间,平均为0.761 5;聚类分析中,在阈值为0.89处可分为2类,第1类主要为牛迭肚野生资源,第2类主要为栽培品种资源,在0.885处又可细分为2个小组,包括国外引进品种资源,国内选育品种(品系)和野生库页悬钩子资源。本研究指纹图谱的构建和遗传多样性分析,为悬钩子属种质资源开展深入研究奠定基础。DNA fingerprinting of 94 Rubus germplasm resources was constructed by using SSR molecular marker technology in which SSR-PCR products were detected through capillary electrophoresis, and then their genetic diversity was analyzed. The results showed that 13 pairs of SSR primers were selected from 40 pairs of primers,with high polymorphism and good stability, and a total of 242 alleles were amplified, with 242 polymorphism loci and a polymorphism rate of 100%. The numbers of detected alleles were between 11~27, with an average of18.6154. The mean values of allele number(Na), effective allele number(Ne), Nei’s gene diversity index(H) and Shannon information index(I) were 2.000 0, 1.104 4, 0.074 8 and 0.139 7, respectively. Two group resources were identified at 0.89, the cluster threshold, according to the cluster analysis. The first group is mainly the wild resources of Rubus crataegifolius, and the second group, mainly cultivated varieties of resources. Moreover, the second group can be further divided into two groups at 0.885, including introduced varieties resources from foreign countries, domestic bred varieties(strains) and wild Rubus sachalinensis. The construction of DNA fingerprinting and the analysis of genetic diversity lay a foundation for the further study of Rubus germplasm resources.

关 键 词：悬钩子 种质资源 DNA指纹图谱 遗传多样性 

分 类 号：S663.2[农业科学—果树学]