宏基因组学揭示鳗鲡养殖环境微生物抗性基因的赋存特征  

Occurrence Characteristics of Antibiotic Resistance Genes in the Culture Environment of Eel Revealed by Metagenomics

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作  者:黄薇 廖茜 罗土炎 宋永康 HUANG Wei;LIAO Qian;LUO Tu-yan;SONG Yong-kang(Institute of Agricultural Quality Standards and Testing Technology, Fujian Academy of AgriculturalSciences/Fujian Key Laboratory of Agro-products Quality & Safety, Fuzhou, Fujian 350003, China;Fujian Institute of Food and Drug Quality Control, Fuzhou, Fujian 350005, China)

机构地区:[1]福建省农业科学院农业质量标准与检测技术研究所/福建省农产品质量安全重点实验室,福建福州350003 [2]福建省食品药品质量检验研究院,福建福州350005

出  处:《福建农业科技》2020年第9期1-5,共5页Fujian Agricultural Science and Technology

基  金:福建省自然科学基金项目(2019J01101);福建省科技计划项目——省属公益类科研院所基本科研专项(2018R1018-1);福建省农业科学院英才项目(YC2019011)。

摘  要:为揭示鳗鲡养殖环境中抗生素抗性基因(Antibiotic resistance genes,ARGs)的赋存特征,采集鳗鲡养殖环境样品(底泥和养殖水),利用高通量测序技术及功能宏基因组学分析方法对鳗鲡养殖环境中的ARGs种类和丰度进行研究。结果显示:鳗鲡养殖底泥和养殖水中的优势菌群结构组成具有明显差异,底泥的优势群体门类为Proteobacteria、Chloroflexi、Actinobacteria、Cyanobacteria、Gemmatimonadetes和Bacteroidetes;养殖水中的优势菌群门类为Proteobacteria、Bacteroidetes和Verrucomicrobia。比对抗性基因数据库,在底泥中共存在10类38种ARGs,在养殖水中共注释得到9类29种ARGs,其中高丰度的抗性类型为四环素类、磺胺类和杆菌肽类抗生素。研究结果可为抗生素在鳗鲡养殖过程中合理使用提供参考依据,同时也显示宏基因组测序技术在检测环境ARGs的可行性和优越性。In order to reveal the occurrence characteristics of antibiotic resistance genes(ARGs)in culture environment of eel,through the collection of the culture environment samples for eel(sediment and aquaculture water),the species and abundance of ARGs in the culture environment of eel were studied by using the analysis methods of high-throughput sequencing technology and functional metagenomics.The results showed that there were significant differences in the structure composition of dominant microflora between the aquaculture sediment and aquaculture water of eel.The dominant microflora genera of sediment included Proteobacteria,Chloroflexi,Actinobacteria,Cyanobacteria,Gemmatimonadetes and Bacteroidetes,while the dominant microflora genera of aquaculture water included Proteobacteria,Bacteroidetes and Verrucomicrobia.Compared with the resistance gene database,there were 38 ARGs in 10 categories in the sediment,and 29 ARGs in 9 categories in the aquaculture water,among which the resistant types with high abundance were tetracyclines,sulfonamides and bacitracin antibiotics.Therefore,the results of this study could provide reference for the rational use of antibiotics during the eel culture,and also show the feasibility and superiority of metagenome sequencing technology in the detection of ARGs in the environment.

关 键 词:鳗鲡 养殖环境 抗生素抗性基因 微生物群落结构 宏基因组学 

分 类 号:S917.1[农业科学—水产科学]

 

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