柴胡桂枝汤对泛耐药鲍曼不动杆菌侵袭的人肺癌A549细胞的保护作用  

Protective Effect of Chaihu Guizhi Decoction on Pan-Drug-Resistant Acinetobacter Baumannii Invading Human Lung Cancer A549 Cells

作　　者：马致洁 于小红[3] 章从恩[3] 马晓晶[2] 王铁山[4] 李慧[5] 林龙飞[5] 赵奎君[3] 黄璐琦[1,2] MA Zhijie;YU Xiaohong;ZHANG Congen;MA Xiaojing;WANG Tieshan;LI Hui;LIN Longfei;ZHAO Kuijun;HUANG Luqi(Center for Post-doctoral Studies,China Academy of Chinese Medical Sciences,Beijing 100700,China;National Resource Center for Chinese Materia Medica,China Academy of Chinese Medical Sciences,Beijing 100700,China;Dept.of Pharmacy,Affiliated Beijing Friendship Hospital of Capital Medical University,Beijing 100050,China;Clinical laboratory center,Affiliated Beijing Friendship Hospital of Capital Medical University,Beijing 100050,China;Institute of Chinese Materia Medica,China Academy of Chinese Medical Sciences,Beijing 100700,China)

机构地区：[1]中国中医科学院博士后科研流动站,北京100700 [2]中国中医科学院中药资源中心,北京100700 [3]首都医科大学附属北京友谊医院药学部,北京100050 [4]首都医科大学附属北京友谊医院临床检验中心,北京100050 [5]中国中医科学院中药研究所,北京100700

出　　处：《中国医院用药评价与分析》2020年第11期1281-1284,1289,共5页Evaluation and Analysis of Drug-use in Hospitals of China

基　　金：“重大新药创制”十三五科技重大专项(No.2019ZX09721001-005-002);中国博士后基金面上项目(No.2018M631704);中央级公益性科研院所基本科研业务费专项课题(No.ZZ10-018-10);中华中医药学会青年人才托举工程项目(No.QNRC2-C13)。

摘　　要：目的:探讨柴胡桂枝汤对泛耐药鲍曼不动杆菌(pan-drug-resistant Acinetobacter baumannii,PDR-AB)侵袭的人肺癌A549细胞的保护作用及可能机制。方法:建立PDR-AB侵袭人肺癌A549细胞模型,采用柴胡桂枝汤进行干预,通过检测细胞存活率、PDR-AB的黏附率和侵袭率,细胞因子肿瘤坏死因子α(TNF-α)、白细胞介素6(IL-6)的浓度以及PDR-AB生物被膜的形成能力等指标,考察柴胡桂枝汤对PDR-AB侵袭的人肺癌A549细胞的保护作用。结果:与模型组相比,柴胡桂枝汤组对PDR-AB侵袭的人肺癌A549细胞有保护作用,可显著提高人肺癌A549细胞存活率(30、15及7.5 mg/ml,P <0.05);显著抑制PDR-AB对A549细胞的黏附作用(柴胡桂枝汤浓度为7.5 mg/ml时,P <0.05;柴胡桂枝汤浓度为30、15 mg/ml时,P <0.01);显著抑制PDR-AB对A549细胞的侵袭作用(柴胡桂枝汤浓度为30 mg/ml时,P <0.05);显著抑制PDR-AB生物被膜的形成(柴胡桂枝汤浓度为15 mg/ml时,P <0.05;柴胡桂枝汤浓度为30 mg/ml时,P <0.01);显著降低TNF-α和IL-6的释放水平(柴胡桂枝汤浓度为30、15 mg/ml时,P <0.01),上述差异均有统计学意义。结论:柴胡桂枝汤对PDR-AB侵袭的人肺癌A549细胞具有一定的保护作用。OBJECTIVE:To probe into the protective effect of Chaihu Guizhi decoction on pan-drug-resistant Acinetobacter baumannii(PDR-AB)invading human lung cancer A549 cells and its possible mechanism.METHODS:A model of PDR-AB invading human lung cancer A549 cells was established,and Chaihu Guizhi decoction was used to conduct intervention.Through detecting cell survival rate,PDR-AB adhesion rate and invasion rate,concentrations of tumor necrosis factorα(TNF-α)and interleukin 6(IL-6)concentration,forming ability of PDR-AB biofilm and other indicators,and the protective effect of Chaihu Guizhi decoction on PDR-AB invading human lung cancer A549 cells was inspected.RESULTS:Compared with the model group,Chaihu Guizhi decoction had protective effect on PDR-AB invading human lung cancer A549 cells,which can significantly improve the survival rate of human lung cancer A549 cells(30,15 and 7.5 mg/ml,P<0.05);significantly inhibit the adhesion of PDR-AB to A549 cells(P<0.05 when the concentration of Chaihu Guizhi decoction was 7.5 mg/ml;P<0.01 when the concentration of Chaihu Guizhi decoction was 30 and 15 mg/ml);significantly inhibit the invasion effect of PDR-AB on A549 cells(P<0.05 when the concentration of Chaihu Guizhi decoction was 30 mg/ml);significantly inhibit the formation of PDR-AB biofilm(P<0.05 when the concentration of Chaihu Guizhi decoction was 15 mg/ml;P<0.01 when the concentration of Chaihu Guizhi decoction was 30 mg/ml);significantly reduce the release levels of TNF-αand IL-6(P<0.01 when the concentration of Chaihu Guizhi decoction was 30 and 15 mg/ml),with statistically significant differences.CONCLUSIONS:Chaihu Guizhi decoction has a certain protective effect on PDR-AB invading human lung cancer A549 cells.

关 键 词：柴胡桂枝汤 PDR-AB 人肺癌A549细胞 

分 类 号：R932[医药卫生—生药学]