黑骨藤醇提取物调控急性痛风性关节炎模型大鼠血清炎性因子及滑膜功能  

Regulating serum inflammatory factors and synovial cell function of gouty arthritis model rats by alcohol extract of Gardneria angustifolia

作　　者：党荣敏[1] 李宁怡 谢洪书[1] 蒋催蓉[1] 罗康华[1] 姚文琴[1] Dang Rongmin;Li Ningyi;Xie Hongshu;Jiang Cuirong;Luo Kanghua;Yao Wenqin(Qiannan Medical College for Nationalities,Duyun 558000,Guizhou Province,China;Research Section of Pain,School of Anesthesiology,Guizhou Medical University,Guiyang 550000,Guizhou Province,China)

机构地区：[1]黔南民族医学高等专科学校,贵州省都匀市558000 [2]贵州医科大学麻醉学院疼痛教研室,贵州省贵阳市550000

出　　处：《中国组织工程研究》2021年第17期2681-2686,共6页Chinese Journal of Tissue Engineering Research

基　　金：黔南民族医学高等专科学校2017校基金项目(QNYZ201704),项目负责人:党荣敏;贵州省厅校联合自然科学类项目(GZWKJ2013-2-003),项目负责人:党荣敏。

摘　　要：背景:研究发现,阻断滑膜分泌炎症递质的药物治疗急性痛风性关节炎有较好的应用前景。近年来,中药治疗急性痛风性关节炎的研究取得了很大成就。目的:探讨黑骨藤醇提取物对急性痛风性关节炎血清炎性因子及滑膜功能的影响。方法:将60只健康SD大鼠随机分为6组,分别为空白对照组、模型对照组、秋水仙碱治疗组、黑骨藤醇提取物高、中、低剂量治疗组,每组10只。除空白对照组外,其他各组均在关节腔内注入尿酸钠溶液制备急性痛风性关节炎模型,然后予以秋水仙碱、高、中、低剂量黑骨藤醇提取物治疗。造模后1-7 d观察关节肿胀度;造模后7 d采用ELISA检测血清和滑膜组织中白细胞介素1β、肿瘤坏死因子α、白细胞介素17水平,苏木精-伊红染色观察滑膜病理改变,Western blot检测滑膜组织中NF-κB、ERK1/2和JNK蛋白表达。结果与结论:①模型对照组大鼠关节肿胀,血清和滑膜组织中白细胞介素1β、肿瘤坏死因子α、白细胞介素17水平升高,滑膜组织具有明显炎性细胞浸润和滑膜细胞增生,NF-κB、ERK1/2和p-JNK蛋白表达水平明显升高;②给予秋水仙碱、黑骨藤醇提取物治疗后,上述指标明显改善,高剂量黑骨藤醇提取物组与秋水仙碱组无显著差异,且黑骨藤醇提取物具有剂量依赖性;③结果表明,黑骨藤醇提取物能明显改善急性痛风性关节炎大鼠关节症状,下调炎症因子表达水平,改善滑膜功能,该作用可能与其下调NF-κB、ERK1/2和p-JNK蛋白表达有关。BACKGROUND:Studies have found that drugs that block the secretion of inflammatory transmitters in the synovium have good application prospects in the treatment of acute gouty arthritis.In recent years,great achievements have been made in the treatment of acute gouty arthritis with traditional Chinese medicine.OBJECTIVE:To study the effect of alcohol extract of Gardneria angustifolia on serum inflammatory factors and synovial cell function in patients with acute gouty arthritis.METHODS:Sixty healthy Sprague-Dawley rats were randomly divided into six groups:blank control group,model control group,colchicine treatment group,Gardneria angustifolia alcohol extract high,medium and low dose treatment groups,withy 10 rats in each group.In addition to the blank control group,the other groups were injected with sodium urate solution into the articular cavity to prepare acute gouty arthritis models,and then treated with colchicine,high,medium and low doses of Gardneria angustifolia alcohol extract.The swelling degree of the joint was observed at 1-7 days after modeling.At 7 days after modeling,the levels of interleukin-1β,tumor necrosis factor-α,interleukin-17 in the serum and synovium were detected by ELISA;the pathological changes of the synovium were observed by hematoxylin-eosin staining,and the expression of nuclear factor-κB,ERK1/2 and JNK protein in the synovium was detected by western blot.RESULTS AND CONCLUSION:In the model control group,the joint swelling,the level of interleukin-1β,tumor necrosis factor-α,and interleukin-17 in the serum and synovium were increased,the synovium had obvious inflammatory cell infiltration and synovium cell proliferation,the expression of nuclear factor-κB,ERK1/2 and p-JNK protein was significantly increased.After the treatment with colchicine and Gardneria angustifolia alcohol extracts,the above indexes were significantly improved,and there was no significant difference in the high dose Gardneria angustifolia alcohol extract group and colchicine group.Moreover,Gardneria angust

关 键 词：骨 痛风性关节炎 黑骨藤醇提取物 炎症 因子 滑膜 大鼠 实验 

分 类 号：R459.9[医药卫生—治疗学] R318[医药卫生—临床医学]