透骨消痛胶囊干预早期骨关节炎滑膜水肿模型大鼠的抗炎消肿作用  

Anti-inflammatory and anti-swelling effects of Tougu Xiaotong Capsule in rat models of early osteoarthritis with synovial edema

作　　者：黄云梅 冯芳芳 陈文列 卢小冬 林如辉 李钻芳 黄美雅 谭春江 李西海 李孝栋[4] Huang Yunmei;Feng Fangfang;Chen Wenlie;Lu Xiaodong;Lin Ruhui;Li Zuanfang;Huang Meiya;Tan Chunjiang;Li Xihai;Li Xiaodong(Institute of Integrated Chinese and Western Medicine,Fujian University of Traditional Chinese Medicine,Fuzhou 350122,Fujian Province,China;National Third-level TCM Laboratory-TCM Pharmacology(Cell Structure and Function)Laboratory,Fuzhou 350122,Fujian Province,China;Fujian Province Key Laboratory of Integrated Chinese and Western Medicine for Geriatric Diseases,Fuzhou 350122,Fujian Province,China;School of Pharmacy,Fujian University of Traditional Chinese Medicine,Fuzhou 350122,Fujian Province,China)

机构地区：[1]福建中医药大学中西医结合研究院,福建省福州市350122 [2]国家中医药三级科研实验室-中药药理(细胞结构与功能)实验室,福建省福州市350122 [3]福建省中西医结合老年性疾病重点实验室,福建省福州市350122 [4]福建中医药大学药学院,福建省福州市350122

出　　处：《中国组织工程研究》2021年第17期2697-2702,共6页Chinese Journal of Tissue Engineering Research

基　　金：福建省自然科学基金项目(2014J01356),项目负责人:陈文列。

摘　　要：背景:前期研究结果显示,透骨消痛胶囊能降低软骨细胞炎症因子表达,减少软骨细胞凋亡,促进软骨细胞增生,延缓软骨、软骨下骨退变,从而缓解骨关节炎疾病进程,减轻炎症反应,但该方对滑膜病变的作用机制尚不明确。目的:从早期骨关节炎滑膜炎症水肿与相关水通道蛋白调节的角度,进一步探讨透骨消痛胶囊抗炎消肿的作用机制。方法:24只SD大鼠随机分成空白组、模型组、中药组3组,后两组应用木瓜蛋白酶法制备早期骨关节炎模型1周后,分别以透骨消痛胶囊(中药组)或生理盐水(空白组和模型组)灌胃4周。小动物MRI观察膝关节形态结构,放射免疫法测定血清和关节液白细胞介素1β和肿瘤坏死因子α水平,石蜡切片观察滑膜组织显微结构,透射电镜观察滑膜细胞超微结构,免疫组化/荧光染色定量、定位水通道蛋白1和水通道蛋白3的表达。实验方案经福建中医药大学动物实验伦理委员会批准(批准号为[2019]福中医伦理审字第024号)。结果与结论:①与模型组相比,中药组滑膜水肿减轻、滑液分泌减少;血清和关节液白细胞介素1β和肿瘤坏死因子α水平明显下降;滑膜细胞增生减少,组织纤维化和血管增生明显减轻;滑膜细胞粗面内质网、高尔基体、囊泡和溶酶体等减少;水通道蛋白1和水通道蛋白3表达显著下调,散在分布于细胞膜和细胞质,无聚集现象;②结果说明,透骨消痛胶囊可能通过减少滑膜水通道蛋白1和水通道蛋白3含量,减少滑液分泌,改善关节水肿,这可能是该药物抗炎消肿,延缓骨关节炎进展的作用机制之一。BACKGROUND:Previous studies have shown that Tougu Xiaotong Capsules can reduce the expression of chondrocyte inflammatory factors,reduce chondrocyte apoptosis,promote chondrocyte proliferation,and delay cartilage and subchondral bone degeneration,thereby alleviating the process of osteoarthritis and reducing inflammation responses.However,the mechanism of this prescription on synovial lesions is unclear.OBJECTIVE:To further explore the mechanism of anti-inflammatory and anti-swelling effect of Tougu Xiaotong Capsule on early osteoarthritis with synovial edema based on the regulation of aquaporins.METHODS:Twenty-four Sprague-Dawley rats were randomly divided into three groups:blank group,model group and traditional Chinese medicine(TCM)group.Osteoarthritis animal models were made by papain injection.After 1 week,animals were gavaged respectively with Tougu Xiaotong Capsule in the TCM group or saline in the blank and model groups for 4 weeks.The morphology and structure of the knee joints were observed by MRI.Interleukin-1βand tumor necrosis factorαin serum and joint fluid were detected by radioimmunoassay.Synovial tissue microstructure was observed through paraffin section,and the ultrastructure of synovial cells observed by transmission electron microscope.Aquaporins 1 and 3 were quantified and located by immunohistochemical staining/fluorescence staining.The study protocol was approved by the Laboratory Animal Ethics Committee of Fujian University of Traditional Chinese Medicine(approval No.[2019]024).RESULTS AND CONCLUSION:Compared with the model group,synovial lesion was alleviated and synovial secretion was decreased in the TCM group;the contents of interleukin-1βand tumor necrosis factorαin the serum and joint fluid were decreased significantly in the TCM group;synovial cell hyperplasia was alleviated,tissue fibrosis and vascular hyperplasia was significantly reduced in the TCM group;the number of rough endoplasmic reticula,Golgi apparatus,vesicles and lysosomes was decreased in synovial cells in the TCM

关 键 词：滑膜 中草药 骨关节炎 滑膜炎症 水通道蛋白 抗炎消肿 超微结构 实验 

分 类 号：R446[医药卫生—诊断学] R496[医药卫生—临床医学]