新型“磁包金”金磁纳米粒子免疫层析试纸条定量检测血清中的人绒毛膜促性腺激素  

Novel Magnet-coated Gold Nanoparticles-based Immunochromatographic Strip for Quantitative Detection of Human Chorionic Gonadotropin in Serum

作　　者：张燚 郝良文 沈轩昂 江湖[2,3] 黄小林 熊勇华[1,2,3] ZHANG Yi;HAO Liang-Wen;SHEN Xuan-Ang;JIANG Hu;HUANG Xiao-Lin;XIONG Yong-Hua(2,31.School of Food Science,Nanchang University,Nanchang 330031,China;State Key Laboratory of Food Science and Technology,Nanchang University,Nanchang 330047,China;Jiangxi-OAI Joint Research Institute,Nanchang University,Nanchang 330047,China)

机构地区：[1]南昌大学食品学院,南昌330031 [2]南昌大学食品科学与技术国家重点实验室,南昌330047 [3]南昌大学中德联合研究院,南昌330047

出　　处：《分析化学》2020年第12期1666-1673,共8页Chinese Journal of Analytical Chemistry

基　　金：“十三五”科技部重点研发计划专项项目(No.2018YFC1602505)资助。

摘　　要：将油酸修饰的氧化铁纳米粒子(Oleic acid coated iron oxide nanoparticles,OC-IONPs)与油胺修饰的金纳米粒子(Oleylamine-coated gold nanoparticles,OA-AuNPs)通过乳液自组装法共封装在聚合物基质中,合成了具有“磁包金”核壳异质结构的新型金磁纳米粒子(Magnetic coated gold nanoparticles,MGNPs)。由于OA-AuNPs聚集在内核,OC-IONPs分布于外壳,有效避免了OA-AuNPs的磁屏蔽效应,相较于传统“金包磁”型纳米结构,此MGNPs具有高的磁饱和强度(为初始氧化铁纳米粒子的80%)和强的吸光度(为传统30 nm胶体金纳米粒子的12.5倍)。进一步以此MGNPs为免疫层析(Immunochromatographic assay,ICA)的新型双功能标记探针,以人绒毛膜促性腺激素(Human chorionic gonadotropin,HCG)为模型检测物,建立了高灵敏检测人血清中HCG的免疫层析方法(MGNPs-ICA)。本研究所构建的试纸条定量检测人血清中HCG的线性范围为0.97~250 mIU/mL(y=0.2561ln x-0.0429,R 2=0.9816),检出限为0.97 mIU/mL;试纸条的批内及批间回收率为93.7%~109.1%,相对标准偏差小于14.3%;特异性实验结果表明,本方法仅对目标物HCG有显著的信号响应。本方法检测结果与化学发光免疫分析(Chemiluminescence immunoassay,CLIA)结果具有较好的一致性(y=1.11 x+14.71,R 2=0.958),但在检测时间、成本和便携性方面具有明显优势。本研究合成的MGNPs具有优异的磁学性能和良好的光学传感性能,能够显著改善免疫层析平台的检测性能。A facile emulsion self-assembly strategy by co-assembling oleylamine-coated gold nanoparticles(OA-AuNPs)with oleic acid-coated iron oxide nanoparticles(OC-IONPs)into polymer nanobeads to form magnetic coated gold nanoparticles(MGNPs)was reported.The synthesized MGNPs exhibited a typical"magnetic-coated gold"core-shell heterostructure.Due to that OA-AuNPs aggregated in core and OC-IONPs assembled in shell,the magnetic shielding effect of OA-AuNPs was effectively avoided.Therefore,compared with the traditional"gold-coated magnetic"nanostructures,the MGNPs synthesized here had higher magnetic saturation intensity(80%of OC-IONPs)and strong absorbance(12.5 times that of the traditional 30 nm colloidal gold nanoparticles).By using MGNPs as the dual-function labeled probe and human chorionic gonadotropin(HCG)as a model biomarker,a highly sensitive immunochromatographic method(MGNPs-ICA)for detecting biomarkers in human serum was established.The results showed that the linear range of the MGNPs-ICA strip for quantitative detection of HCG in human serum was 0.97-250 mIU/mL,and the linear regression equation was y=0.2561ln x-0.0429(R 2=0.9816).The detection limit was 0.97 mIU/mL.The intra-and inter-assay recoveries for HCG spiked serum were 93.7%-109.1%,and the coefficient of variation was less than 14.3%.The specific experimental results showed that the method only showed a significant signal response to target HCG.In addition,the results of this method were in good agreement with those of chemiluminescence immunoassay(CLIA).Compared with CLIA method,MGNPs-ICA method had significant advantages in detection time,cost and portability.In a word,the as-prepared MGNPs had excellent magnetic properties and strong optical sensing ability,and could significantly improve the detection performance of ICA platform.

关 键 词：磁包金纳米粒子 免疫层析试纸条 人绒毛膜促性腺激素 定量检测 

分 类 号：R446.6[医药卫生—诊断学]