基于络合反应纳米自聚集的荧光/表面增强拉曼光谱双传感模式检测锌离子  

Fluorescence and Surface Enhanced Raman Scattering Dual Modal Detection of Zn^2+Based on Complexation Reaction-Induced Self-Aggregation of Nanoparticles

作　　者：郑思倾 李丹 邓维 司文帅[2] 白冰[2] ZHENG Si-Qing;LI Dan;DENG Wei;SI Wen-Shuai;BAI Bing(School of Chemical and Environmental Engineering,Shanghai Institute of Technology,Shanghai 201418,China;Institute for Agro-Food Standards and Testing Technology,Shanghai Academy of Agricultural Sciences,Shanghai 201403,China)

机构地区：[1]上海应用技术大学化学与环境工程学院,上海201418 [2]上海市农业科学院农产品质量标准与检测技术研究所,上海201403

出　　处：《分析化学》2020年第12期1687-1693,I0005-I0011,共14页Chinese Journal of Analytical Chemistry

基　　金：国家自然科学基金项目(Nos.21507089,21976123);上海市科学技术委员会农业领域项目(No.19391901800)资助~~

摘　　要：纳米光学探针具有独特的结构特征和光学特性,受到广泛的关注。本研究合成了一种新的荧光/表面增强拉曼散射(SERS)双模式纳米光学探针,利用羟基苯甲酸衍生物(NAMH)的酚羟基和席夫碱对Zn^2+具有优良亲和力的特性,构建了NAMH修饰银纳米颗粒(AgNPs@NAMH)探针,AgNPs@NAMH与Zn^2+之间发生特异的络合作用,诱导荧光信号猝灭,可用于复杂样本中Zn 2+的荧光检测,检出限为0.32 nmol/L。此外,络合反应引发AgNPs发生自聚集,可实现对Zn^2+的超灵敏SERS检测,检出限为0.68 pmol/L。本方法具有操作简单、快速、高灵敏和高选择性等优点,为环境污染物的即时检测提供了技术支持。Optical nanoprobes have attracted extensive attention in area of analytical chemistry because of their unique structural characteristics and optical properties.In this study,a novel fluorescence/surface-enhanced Raman scattering(SERS)dual-modal nanoprobe was developed.The phenolic hydroxyl group and Schiff base of hydroxybenzoic acid derivative(NAMH)exhibited a preferential affinity towards Zn^2+.The complexation between AgNPs@NAMH and Zn^2+induced the quenching of the fluorescent signal of NAMH,thus enabling the fluorescent detection of Zn^2+in complex samples with a detection limit of 0.32 nmol/L.Moreover,the Zn^2+-triggered AgNPs aggregation led to the formation of abundant“hot spots”among NPs,which could be used for SERS analysis of Zn^2+with a detection limit of 0.68 pmol/L.This method had a number of advantages over conventional approaches in simplicity of operation,rapidity,high sensitivity and high selectivity,and provided new technical support for the immediate detection of environmental pollutants.

关 键 词：荧光 表面增强拉曼光谱 络合反应 锌离子 

分 类 号：X832[环境科学与工程—环境工程] O657.3[理学—分析化学]