表面增强拉曼光谱同时检测原烟中三唑醇和三唑酮残留  

Simultaneous Determination of Triadimenol and Triadimefon in Raw Tobacco by Surface-enhanced Raman Spectroscopy

作　　者：李霞[1] 陈晓水[1] 黄艺伟 杨君[1] 温宝英 周国俊[1] 李剑锋[2] LI Xia;CHEN Xiao-Shui;HUANG Yi-Wei;YANG Jun;WEN Bao-Ying;ZHOU Guo-Jun;LI Jian-Feng(China Tobacco Zhejiang Industrial Co.Ltd.,Hangzhou 310008,China;College of Chemistry and Chemical Engineering,Xiamen University,Xiamen 361005,China)

机构地区：[1]浙江中烟工业有限责任公司,杭州310008 [2]厦门大学化学化工学院,厦门361005

出　　处：《分析化学》2020年第12期1747-1752,共6页Chinese Journal of Analytical Chemistry

基　　金：国家自然科学基金项目(Nos.21925404、21775127);浙江中烟工业有限责任公司科技项目(No.ZJZY2019C005)资助。

摘　　要：基于表面增强拉曼光谱(SERS)可在低浓度待测物质甚至是单分子水平上获得高灵敏度结构信息的优势,本研究提出并建立了一种结合便携式拉曼光谱仪,利用SERS实现原烟中广谱杀菌剂三唑醇和三唑酮残留同时快速检测的方法。采用酸性条件下的聚酰胺作为净化剂,以除去烟草中色素、酚类、有机酸类等的干扰影响,以纳米金溶胶作为SERS基底,通过三唑醇或三唑酮在金溶胶表面的吸附放大拉曼信号,实现高灵敏检测。结果表明,原烟中三唑醇、三唑酮残留量的检测灵敏度为1 mg/kg,检测时间为8~12 min。本研究建立的SERS检测方法操作简便、成本低、快速、准确,适用于烟草中三唑醇和三唑酮农药残留的快速检测。Surface-enhanced Raman spectroscopy(SERS)can obtain highly sensitive structural information of substances with ultralow concentration or even at the single-molecule level.Herein,a rapid detection method for triadimenol and triadimefon in raw tobacco was established using SERS combined with portable Raman spectrometer.In this method,polyamide under acidic conditions was used as a purifying agent to remove the interference from pigments,phenols,organic acids and other impurities in tobacco.Gold-based SERS substrates were used to amplify the Raman signals through the adsorption of triadimenol and triadimefon on the surface of gold nanoparticles.It was found that the detection sensitivity of triadimenol and triadimefon residues in raw tobacco was 1 mg/kg,and the time needed was 8-12 min.This method was simple,low-cost,and accurate,thus was suitable for the rapid detection of triadimenol and triadimefon pesticide residues in tobacco.

关 键 词：表面增强拉曼光谱 烟草 农药残留 三唑醇 三唑酮 聚酰胺 快速检测 

分 类 号：O657.37[理学—分析化学] TS411[理学—化学]