萝卜病毒病小RNA分析及资源抗性表现  

Small RNA Analysis and Resource Resistance Expression of Radish Virus Disease

作　　者：甘彩霞[1,2] 张兴中 崔磊[1,2] 於校青[1,2] 王飞 戴照义 严承欢[1,2] 邓晓辉 姚明华[3] 邱正明 焦春海 GAN Caixia;ZHANG Xingzhong;CUI Lei;YU Xiaoqing;WANG Fei;DAI Zhaoyi;YAN Chenghuan;DENG Xiaohui;YAO Minghua;QIU Zhengming;JIAO Chunhai(Institute of Cash Crops,Hubei Academy of Agricultural Sciences,Wuhan 430070,Hubei,China;Hubei Key Laboratory of Vegetable Germplasm Innovation and Genetic Improvement,Wuhan 430070,Hubei,China;Hubei Academy of Agricultural Sciences,Wuhan 430064,Hubei,China)

机构地区：[1]湖北省农业科学院经济作物研究所,湖北武汉430070 [2]蔬菜种质创新与遗传改良湖北省重点实验室,湖北武汉430070 [3]湖北省农业科学院,湖北武汉430064

出　　处：《中国蔬菜》2020年第12期28-34,共7页China Vegetables

基　　金：国家重点研发计划项目(2017YFD0101806,2016YFD0100204-19);湖北省重点研发计划项目(2020BBA037);湖北省农业科技创新中心创新团队项目(2019-620-000-001-01)。

摘　　要：病毒病是为害萝卜的主要病害之一。基于核酸检测的植物病毒病检测技术,因其检测范围广、灵敏度高、适合大批量检测而得以广泛应用。本试验利用小RNA高通量测序方法鉴定萝卜病毒病的种类,并调查分析了264份萝卜核心种质资源及育种品系对病毒病的抗性。结果表明:萝卜病毒病的主要病原为芜菁花叶病毒(TuMV)。参试的核心种质资源和品系多数表现出较好的抗性,且不同品系姊妹系间抗性表现基本趋于一致。田间抗病性调查结果显示:抗病材料有103份,高抗材料有64份,且有25份材料达到免疫。Virus disease is one of the main diseases harming radish.Plant virus disease detection technology based on nucleic acid detection has been widely used owing to its wide detection range,high sensitivity and suitability for mass detection.Adopting small RNA high-throughput sequencing method,this experiment identified the kinds of radish virus disease,investigated and analyzed 264 accessions of radish core germplasm resources and breeding lines resistance to virus diseases.Results indicated that the major pathogeny of radish virus disease was turnip mosaic(TuMV).Most of the tested core germplasm resources and breeding lines expressed better resistance performance,and resistance between different breeding lines and sister lines basicly verge to have no difference.Results of field investigation on resistance showed that there were 103 accessions with disease-resistance,of which 64 were high-resistant and 25 were immune.

关 键 词：萝卜 病毒病 小RNA高通量测序 种质资源 品系 抗病性 

分 类 号：S436.31[农业科学—农业昆虫与害虫防治]