强直性脊柱炎患者髓源抑制细胞浸润和血清细胞因子表达的研究  

Changes in myeloid-derived suppressor cells and cytokines in peripheral blood of patients with ankylosing spondylitis

作　　者：应泳丽 陈佳琦[2] 曲春生[2] 兰丽康[3] Ying Yongli;Chen Jiaqi;Qu Chunsheng;Lan Likang(Operation Room,Lishui People's Hospital,Lishui,Zhejiang 323000,China;Department of Clinical Laboratory,Lishui People's Hospital,Lishui,Zhejiang 323000,China;Department of Neurology,Lishui People's Hospital,Lishui,Zhejiang 323000,China)

机构地区：[1]丽水市人民医院手术室,浙江省323000 [2]丽水市人民医院检验科,浙江省323000 [3]丽水市人民医院神经内科,浙江省323000

出　　处：《中国基层医药》2020年第21期2618-2621,共4页Chinese Journal of Primary Medicine and Pharmacy

基　　金：浙江省中医药管理局基金项目(2019ZA132);浙江省丽水市医学重点学科建设项目(2017ZDXK03)。

摘　　要：目的研究强直性脊柱炎(AS)患者外周血中髓源抑制细胞(MDSCs)和细胞因子的表达。方法选取丽水市人民医院2018年6月至2019年6月收治的AS患者50例(AS组)和同期健康体检者50例(对照组),运用流式细胞术检测两组外周血多核型髓源抑制性细胞(PMN-MDSC)、单核型髓源抑制性细胞(M-MDSC)数量及其细胞因子白细胞介素2(IL-2)、白细胞介素4(IL-4)、白细胞介素6(IL-6)、白细胞介素10(IL-10)、肿瘤坏死因子α(TNF-α)、γ-干扰素(γ-IFN)、白细胞介素17(IL-17)表达。分析MDSCs表达在两组中的差异及其与细胞因子表达的关系。结果AS组外周血中PMN-MDSC、M-MDSC、IL-6分别为(0.22±0.08)%、(1.48±0.32)%、(20.74±5.14)ng/L,均显著高于对照组的(0.06±0.02%)%、(0.43±0.11%)%、(2.52±0.53)ng/L,差异均有统计学意义(t=7.933、6.310、10.553,均P<0.05)。结论IL-6可能是促进MDSCs募集与浸润的原因之一,MDSCs增高可能与AS感染后免疫应答及疾病发生、发展相关。Objective To investigate the expression of myeloid-derived suppressor cells(MDSCs)and cytokines in peripheral blood of patients with ankylosing spondylitis(AS).Methods From June 2018 to June 2019,50 AS patients(AS group)and 50 healthy people(control group)in Lishui People's Hospital were selected in this study.The number of PMN-MDSC and monocyte MDSC(M-MDSC)and the expression of cytokines(IL-2,IL-4,IL-6,IL-10,TNF-α,γ-IFN and IL-17)in the peripheral blood were detected by flow cytometry.The differences of MDSCs expression frequency in each group and its relationship with cytokine expression were analyzed.Results The PMN-MDSC,M-MDSC and IL-6 in peripheral blood of the AS group were(0.22±0.08)%,(1.48±0.32)%and(20.74±5.14)ng/L,respectively,which were significantly higher than those of the control group[(0.06±0.02)%,(0.43±0.11)%and(2.52±0.53)ng/L],and the differences were statistically significant(t=7.933,6.310,10.553,all P<0.05).Conclusion IL-6 may be one of the reasons to promote the infiltration and recruitment of MDSCs,and the increase of MDSCs may be related to the occurrence and development of immune tolerance after ankylitis infection.

关 键 词：脊柱炎 强直性 髓源抑制细胞 多核型髓源抑制性细胞 单核型髓源抑制性细胞 白细胞介素4 白细胞介素6 白细胞介素10 肿瘤坏死因子-α γ-干扰素 

分 类 号：R593.23[医药卫生—内科学]