外源茉莉酸甲酯诱导番茄对烟粉虱的抗虫性  

Insect resistance of tomato induced by exogenous methyl jasmonate to Bemisia tabaci

作　　者：张晓霞 洪波[1] 景亮亮 曹爽 贾彦霞[1] ZHANG Xiao-xia;HONG Bo;JING Liang-liang;CAO Shuang;JIA Yan-xia(College of Agronomy,Ningxia University,Yinchuan 150021,China)

机构地区：[1]宁夏大学农学院,银川750021

出　　处：《生态学杂志》2020年第11期3651-3657,共7页Chinese Journal of Ecology

基　　金：宁夏“十三五”重点研发计划重大项目(2018BBF02021-02)资助。

摘　　要：采用盆栽法研究茉莉酸甲酯(MeJA)处理后番茄叶片营养物质含量、次生代谢物含量和防御酶活性,以及MeJA处理对烟粉虱成虫的防治效果。结果表明:外源MeJA可以诱导番茄叶内营养物质含量的降低,用0.0225 mmol·L^-1的MeJA处理5 d后,番茄叶片中可溶性糖和蛋白质含量最低,诱导效应最强;外源MeJA可诱导番茄叶内次生代谢产物含量的升高,用0.0225 mmol·L^-1的MeJA处理5 d后,番茄叶中单宁和黄酮含量最高,诱导效应最强;外源MeJA能够诱导番茄叶内防御性酶(POD和PPO)活性升高,随着时间的延长(0~14 d),不同浓度MeJA处理后防御酶活性呈现出先上升后下降再上升的变化趋势,且用0.0225 mmol·L^-1的MeJA处理5 d后,番茄叶中POD和PPO活性最大,诱导效应最强;不同浓度的外源MeJA对烟粉虱的防效不同,用0.0225 mmol·L^-1MeJA处理5 d后,对烟粉虱的防治效果最好,防效可达86.96%;外源茉莉酸甲酯处理可以通过降低营养物质含量、提高次生代谢产物含量以及蛋白防御酶活性来增加番茄对烟粉虱的抗性,研究结果为防治烟粉虱提供科学依据。To clarify the roles of exogenous methyl jasmonate(MeJA) in controlling Bemisia tabaci adults on tomatoes,we measured nutrient content,secondary metabolite content and protein defense enzyme activity in tomato leaves under MeJA treatments in a pot experiment. The results showed that exogenous MeJA treatment reduced nutrient content in tomato leaves. The soluble sugar and protein contents were the lowest and the induction effect was the strongest under the treatment of 0.0225 mmol·L^-1 MeJA after five days. The exogenous MeJA treatment increased the secondary metabolites in tomato leaves,with the strongest induction effect and the highest concentrations of tannin and flavonoid being observed in tomato leaves under the MeJA treatment with application rate of 0.0225 mmol·L^-1 after five days. The exogenous MeJA enhanced the activity of defense enzymes(POD and PPO) in tomato leaves. With the extension of MeJA treatment time(0-14 days),the activity of defense enzymes showed a trend of increasing-decreasingincreasing. The POD and PPO activities in tomato leaves were the highest and the induction effect was the strongest at the treatment of 0.0225 mmol·L^-1 MeJA after five days. Different concentrations of the exogenous MeJA application had different control effects on B. tabaci. After five days treatment with 0.0225 mmol·L^-1 MeJA,the best control effect on B. tabaci was observed,reaching 86.96%. In conclusion,the application of exogenous MeJA can increase the resistance of tomato to B. tabaci through reducing nutrient content,increasing secondary metabolite content and protein defense enzyme activity. Our results provide scientific basis for the prevention of B. tabaci in tomato planting and management.

关 键 词：茉莉酸甲酯 番茄 烟粉虱 诱导抗虫性 

分 类 号：S436.412.2[农业科学—农业昆虫与害虫防治]