白藜芦醇减轻脑创伤大鼠炎性反应及神经元凋亡  

Resveratrol alleviates inflammatory response and neuron apoptosis in rats with traumatic brain injury

作　　者：简宇[1] 代凌云[1] 吴丰学[1] JIAN Yu;DAI Ling-yun;WU Feng-xue(Department of Emergency, Jingzhou Central Hospital, Jingzhou 443000, China)

机构地区：[1]荆州市中心医院急诊科,湖北荆州443000

出　　处：《基础医学与临床》2020年第12期1631-1635,共5页Basic and Clinical Medicine

基　　金：湖北省卫计委基金(WJ2015MB121)。

摘　　要：目的研究白藜芦醇对脑创伤(TBI)大鼠炎性反应及神经元凋亡的影响。方法将大鼠分为对照组(control)、模型组(用液压打击法构建TBI大鼠模型)、白藜芦醇(RV)低、中和高剂量处理组(于建模前30 min分别舌下静脉注射5、10、20 mg/kg RV),每组8只;观察各组大鼠创伤后空间学习和记忆能力、海马区神经元形态、神经元凋亡、炎性因子水平及凋亡相关蛋白的变化。结果与对照组相比,TBI组大鼠运动轨迹、搜索安全岛潜伏期明显增加,海马神经元损伤显著,神经元凋亡率、caspase-3、Notch1及Hes1的表达明显上升,Bcl-2/Bax水平明显下降(P<0.05);与TBI组相比,RV可呈剂量依赖性缩短大鼠运动轨迹、搜索安全岛潜伏期,减轻海马神经元损伤,下调神经元凋亡率、caspase-3、Notch1及Hes1的表达,并上调Bcl-2/Bax水平(P<0.05)。结论白藜芦醇可有效减轻脑创伤大鼠神经元损伤及炎性反应,抑制神经元的凋亡,可能与下调Notch1和Hes1的表达有关。Objective To explore effects of resveratrol(RV)on inflammatory response and neuron apoptosis in rats with traumatic brain injury(TBI).Methods The rats were divided into control group(control),model group(constructing TBI rat model by fluid-percussion method),low,medium and high-dose RV groups with 8 animals in each.The changes of spatial learning and memory ability,hippocampal neuronal morphology,neurocyte apoptosis,inflammatory factors level and the presence of apoptosis-related proteins in each group after trauma were observed.Results Compared with control group,motion trajectory and latency of searching safety island were significantly prolonged in TBI group,hippocampal neuron damage was significant,neuron apoptosis rate,expression of caspase-3,Notch1 and Hes1 were significantly increased,and Bcl-2/Bax level was significant decreased(P<0.05).Compared with TBI group,RV could shorten rat motion trajectory with dose-dependence,latency of searching safety island,alleviateneuron damage in hippocampus,down-regulate neuron apoptosis rate,expression of caspase-3,Notch1 and Hes1,and up-regulate Bcl-2/Bax level.Conclusions RV can effectively alleviate neuron damage and inflammatory response of TBI rats,and inhibit neuron apoptosis,which may be relatedto the down-regulating of Notch and Hes1 expression.

关 键 词：白藜芦醇 NOTCH信号通路 脑创伤大鼠 炎性反应 凋亡 

分 类 号：R651.15[医药卫生—外科学]