流式核酸单分子计数方法研究进展  

Research Progress on the Flow Cytometry Counting for Single Nucleotide Molecule

作　　者：王迪 吴枭 王志栋 高运华[1] WANG Di;WU Xiao;WANG Zhidong;GAO Yunhua(Center for Advanced Measurement Science,National Institute of Metrology,Beijing 100029,China)

机构地区：[1]中国计量科学研究院前沿计量科学中心,北京100029

出　　处：《生物技术进展》2020年第6期573-578,共6页Current Biotechnology

基　　金：国家质量研发重点专项项目(2017YFF0204604);中国计量科学研究院基本科研业务费项目(31-AKY1950)。

摘　　要：研发精确灵敏的核酸定量技术,提升对微量样本的测量能力,在当前抗击新冠疫情的背景下尤其重要。流式核酸单分子计数是一种基于“计数”技术的定量测量方法,该方法无需聚合酶链式反应而对荧光标记的靶分子直接定量,测量结果直接溯源到国际基本单位。作为潜在的基准计量方法,它是当前核酸定量测量技术的重要补充,可广泛应用于临床诊断、转基因标识、食品药品残留检测等多个领域。对流式核酸单分子计数方法的原理、检测体系的硬件组成以及数据分析模型进行了综述,并展望了该方法对构建生物计量标准体系的重要意义。Developing accurate and sensitive nucleotide quantification method to improve the detection capability for traceable nucleotide samples,is of great importance under the background of fighting against present COVID-19 epidemic.Flow cytometry counting for single nucleotide molecule is a type of numeration-based quantification method,which counts the copy number of target fluorescence labelled-nucleotide molecules without PCR amplification,tracing directly to international system of unit.As a potential primary metrological method,it is an important supplement for the present nucleotide quantification method,and could be widely applied for many fields,such as clinical diagnostics,genetic-modified organism labelling and detection of food&drug residues.Here the principal,hardware constitution of the apparatus,data analysis model were reviewed,and the significance to biometric standard system was also prospected.

关 键 词：计量学 流式细胞术 激光诱导荧光检测 数字聚合酶链式反应 

分 类 号：Q52[生物学—生物化学]