冷冻干燥对人类体液代谢组影响的NMR研究  

Effects of Lyophilization on the Metabonomic Phenotypes of Human Biofluids Characterized with NMR Analysis

作　　者：刘思[1,2] 安艳捧 唐惠儒 LIU Si;AN Yan-peng;TANG Hui-ru(State Key Laboratory of Magnetic Resonance and Atomic and Molecular Physics,National Center for Magnetic Resonance in Wuhan(Wuhan Institute of Physics and Mathematics,Chinese Academy of Sciences),Wuhan 430071,China;University of Chinese Academy of Sciences,Beijing 100049,China;State Key Laboratory of Genetic Engineering,Zhongshan Hospital and School of Life Sciences,Human Phenome Institute,Metabonomics and Systems Biology Laboratory at Shanghai International Centre for Molecular Phenomics,Fudan University,Shanghai 200438,China)

机构地区：[1]波谱与原子分子物理国家重点实验室,武汉磁共振中心(中国科学院武汉物理与数学研究所),湖北武汉430071 [2]中国科学院大学,北京100049 [3]遗传工程国家重点实验室,复旦大学生命科学学院,附属中山医院,人类表型组研究院,上海200438

出　　处：《波谱学杂志》2020年第4期484-489,共6页Chinese Journal of Magnetic Resonance

基　　金：国家重点研究计划资助项目(2017YFC0906800);国家自然科学基金资助项目(81590953,31821002,21405020).

摘　　要：样品前处理是代谢组分析的重要环节,而冷冻干燥是样品前处理过程中去除溶剂的重要方法,但冷冻干燥对代谢组分析结果的影响还有待进一步研究.本文以人类尿液和血清样本为研究对象,采用核磁共振(NMR)技术分析了冷冻干燥对这两种重要体液代谢组的影响,发现冷冻干燥会引起体液样本中部分有机羧酸和氨基酸含量的改变;而且,冷冻干燥对尿液和血清样品代谢组的影响不同,这可能与两种体液的生物基质差异有关.本研究结果表明,在代谢组学研究中要格外关注冷冻干燥对样品前处理及后续数据归一化所产生的影响.Sample pretreatment is essential for metabonomic analysis.Lyophilization(freeze-drying)is widely used for sample pretreatment to remove solvent,especially water,prior to nuclear magnetic resonance(NMR)and/or mass spectrum(MS)analysis.However,the effects of lyophilization on metabonomic phenotypes remain to be fully investigated.In this study,NMR analysis was carried out to reveal the lyophilization-induced changes in metabonomic phenotypes of human urinary and serum samples.It was found that lyophilization resulted in large changes in the levels of some carboxylic acids and amino acids.Such effects were different between the urine and serum samples,perhaps indicating a biological matrix-dependent effect.The results of this study suggest that extra care and considerations should be taken when lyophilization is used for sample pretreatment and normalization in metabonomic analysis.

关 键 词：核磁共振(NMR) 冷冻干燥 样品前处理 体液 代谢组学 

分 类 号：O482.53[理学—固体物理]