一种基于多孔硅光子器件的免标记生物检测法  

A label free biological detection method based on porous silicon photonic devices

作　　者：何蕾[1] 贾振红[2] 李传喜 HE Lei;JIA Zhen-hong;LI Chuan-xi(School of Physical Science and Technology,Xinjiang University,Urumqi 830046,China;School of Information Science and Engineering,Xinjiang University,Urumqi 830046,China)

机构地区：[1]新疆大学物理科学与技术学院,新疆乌鲁木齐830046 [2]新疆大学信息科学与工程学院,新疆乌鲁木齐830046

出　　处：《光电子．激光》2020年第10期1013-1017,共5页Journal of Optoelectronics·Laser

基　　金：国家自然科学基金项目:《基于多孔硅光子器件荧光增强的高灵敏度生物检测新方法研究》(61575168)资助项目。

摘　　要：本文提出了一种免标记、快速便捷、低成本、较高灵敏度的生物检测的新方法,其检测原理是通过测量多孔硅中因生物反应引起的折射率的变化来实现对生物分子的检测。文中采用单层多孔硅样品作为传感器基底,将包虫病(EgP38)抗原链接到多孔硅中,再在样品上滴加不同浓度的EgP38抗体分子,抗原与抗体发生特异性反应后,采用椭圆偏振仪检测样品折射率的变化量,通过分析传感器中因发生生物反应而导致的折射率变化量,从而实现生物检测。该方法首次采用椭圆偏振法实现了对包虫病生物分子的检测,对抗体分子的检测限为142 nM。In this paper,a label free,fast,convenient,low-cost and high-sensitivity biological detection method is proposed.The method takes advantage of the optical properties of porous silicon photonic devices,and directly measured the change of refractive index of samples by ellipsometry,a single-layer porous silicon sample is used as the sensor substrate,the echinococcosis(Egp38)antigen was linked to porous silicon,then different concentrations of Egp38 antibody molecules were added to the samples,after specific reaction between antigen and antibody,the change of refractive index was measured by ellipsometry,studied the relationship between the degree of biological reaction and the change of refractive index in the sensor,then realized the biological detection.The detection limit of hydatidosis is 142 nM.

关 键 词：多孔硅 折射率 生物传感器 

分 类 号：O649.5[理学—物理化学]