山东汉族人群血小板抗原基因多态性分析  

Analysis on Polymorphism of Platelet Antigen Gene in Shandong Han Population

作　　者：刘艳[1] 朱海峰[1] 乔文本 张毅[1] 宋永红[1] 聂向民[1] 朱传福[1] 翟培聪 LIU Yan;ZHU Hai-Feng;QIAO Wen-Ben;ZHANG Yi;SONG Yong-Hong;NIE Xiang-Min;ZHU Chuan-Fu;ZHAI Pei-Cong(HLA Laboratory,Blood Center of Shandong Province,Jinan 250014,Shandong Province,China)

机构地区：[1]山东省血液中心HLA研究室,山东济南250014

出　　处：《中国实验血液学杂志》2020年第6期2066-2070,共5页Journal of Experimental Hematology

摘　　要：目的:探讨山东汉族人类血小板抗原(human platelet antigen,HPA)1-17、人类白细胞抗原(human leukocyte antigen,HLA)A、B多态性分布。方法:对山东地区962位无血缘关系的汉族血小板自愿捐献者采用PCR-SSP方法进行HPA1-17系统的基因分型,采用PCR-SSOP技术对HLA-A、B位点进行基因分型,直接计数法计算基因频率,HPA1-17及HLA基因型组合用Arelequin 3.5进行分析。结果:HPA各等位基因频率分别是1a:0.9918,1b:0.0082,2a:0.9419,2b:0.0592,3a:0.5841,3b:0.4174,4a:0.9969,4b:0.0031,5a:0.9892,5b:0.0108,6a:0.9835,6b:0.0175,7-14a:1,7-14b:0,15a:0.5488,15b:0.4512,16a:1,16b:0,17a,17b:0;频率最高的HPA基因组合型为:HPA-(1,2,4,5,6,7-14,16,17)aa-3ab15ab(0.2048);HLA-A、B位点中频率最高的基因分别为A*2(0.3094)、B*13(0.1513),频率最高的HLA基因组合型为:HLA-A*02~B*13(0.1397)。结论:山东汉族人群HPA和HLA分布具有明显多态性,与其他人群相比显示出地域和种族性差异,应建立本地区的血小板捐献者HPA和HLA分型资料库。Objective:To study the Polymorphism of the human platelet antigen(HPA)gene 1-17 and human leukocyte antigen(HLA)gene-A and B locus in Shandong Han population.Methods:A total of 962 samples from routine voluntary platelet donors were genotyped for HPA1-17 system and HLA-A site,B by PCR-SSP and PCR-SSOP respectively.Gene frequencies were calculated by counting.HPA1-17 and HLA genotype combinations were analyzed by Arelequin 3.5.Results:The gene frequencies of HPA-la,-1b,HPA-2a,-2b,HPA-3a,-3b,HPA-4a,-4b,HPA-5a,-5b,HPA-6a,-6b,HPA-15a,-15b were 0.9918,0.0082,0.9419,0.0592,0.5841,0.4174,0.9969,0.0031,0.9892,0.0108,0.9835,0.0175,0.5488 and 0.4512,respectively.The most common HPA genotype combination was HPA-(1,2,4,5,6,7-14,16,17)aa-3ab-15ab(0.2048).Moreover,HLA-A*2(0.3094)and HLA-B*13(0.1513)showed the highest frequency in their respective locus.The most common HLA genotype combination was HLA-A*2-B*13(0.1397).Conclusion:Distributions of HPA and HLA show high polymorphism in Shandong Han population.The ethnic and territorial difference of HPA distribution is also confirmed.It is imperative to establish local genetic database of volunteer platelet donors.

关 键 词：人类血小板抗原 人类白细胞抗原 多态性 

分 类 号：R457.1[医药卫生—治疗学]