一株广西鸡源H6亚型禽流感病毒全基因组序列分析及其重要生物学特性  

Whole Genome Sequence Analysis and Important Biological Characteristics of a Strain of H6 Subtype Avian Influenza Virus from Chicken in Guangxi

作　　者：李孟 谢芝勋 李丹 罗思思 谢丽基 张民秀 黄娇玲 范晴 王盛 谢志勤 邓显文 曾婷婷 张艳芳 LI Meng;XIE Zhi-xun;LI Dan(Guangxi Veterinary Research Institute,Guangxi Key Laboratory of Veterinary Biotechnology,Nanning,Guangxi 530001)

机构地区：[1]广西壮族自治区兽医研究所,广西兽医生物技术重点实验室,广西南宁530001

出　　处：《安徽农业科学》2020年第23期125-128,142,共5页Journal of Anhui Agricultural Sciences

基　　金：广西科技重大专项(桂科AA17204057);广西科技基地和人才专项项目(桂科AD17195083);广西“八桂学者”专项(2019-79);国家“万人计划”领军人才专项(2016-37-88)。

摘　　要：2017年对广西地区活禽交易市场进行低致病性禽流感流行病学调查,从采集的鸡咽喉和泄殖腔拭子中分离鉴定出一株鸡源H6N6亚型禽流感病毒,命名为A/chicken/Guangxi/286C10/2017(H6N6)。应用RT-PCR方法分别对分离毒株的8个基因片段进行扩增,同时将产物进行克隆和测序,然后对测序结果进行同源性比对及遗传进化分析。另外,还通过固相受体结合试验对毒株的唾液酸受体进行测定。结果显示,该分离株的HA基因与广东分离株A/chicken/Guangdong/G3249/2014(H6)的核苷酸同源性最高,裂解位点符合低致病性禽流感病毒的分子特征;NA基因与江西分离株A/duck/Ganzhou/GZ148/2016(H6N6)核苷酸同源性最高;内部基因中,PB1和NS基因来源于广东的鸡,PB2基因来源于越南的鸡,PA、NP和M基因来源于广西的鹅;同时,研究还发现该病毒的HA、NA、PA、NP和M基因来源H6亚型AIV,PB2、PB1和NS基因来源H9N2亚型AIV,推测A/chicken/Guangxi/286C10/2017(H6N6)可能是由不同地区、不同宿主来源的H6和H9亚型AIV通过基因重组产生的一株新的重配毒株。由于广西地理位置特殊,今后应加强对该地区H6亚型禽流感病毒变异情况的监测。In 2017,an epidemiological investigation of low-pathogenicity avian influenza was conducted in the live poultry trading market of Guangxi.One strain of H6N6 subtype of avian influenza virus was isolated and identified from throat and cloacal swab samples of chicken,named A/chicken/Guangxi/286C10/2017(H6N6).RT-PCR was used to amplify 8 gene fragments of isolated strains,and the products were cloned and sequenced at the same time.Then homology comparison and genetic evolution analysis were performed on the sequencing results.In addition,the sialic acid receptor of the strain was determined by solid phase receptor binding test.The results showed that HA gene in the isolated strain from Guangdong had the highest nucleotide homology with that in A/chicken/Guangdong/G3249/2014(H6),and the cracking sites conformed to the molecular characteristics of low pathogenic avian influenza virus.NA genes in the isolated from this strain had the highest nucleotide similarity with that in isolated strain A/duck/Ganzhou/GZ148/2016(H6N6).Among internal genes,PB1 and NS genes were derived from chicken in Guangdong,PB2 gene was derived from chicken in Vietnam,and PA,NP and M genes were derived from geese in Guangxi.At the same time,we found that the HA,NA,PA,NP and M genes in this virus were from H6 subtype of AIV,PB2,PB1 and NS genes were from H9N2 subtype of AIV,and it was speculated that A/chicken/Guangxi/286C10/2017(H6N6)might be a new strain of H6 and H9 subtypes of AIV from different regions,different host sources by gene recombination.Due to special geographical location of Guangxi,the monitoring of the variation of H6 subtype avian influenza virus in this region should be strengthened in the future.

关 键 词：禽流感病毒 H6亚型 鸡 遗传进化 受体分析 

分 类 号：S852.65[农业科学—基础兽医学]