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作 者:袁艺[1] 王丹 孙宝新 张洪恩 张雨飞 YUAN Yi;WANG Dan;SUN Bao-xin(College of Life Science,Anhui Agricultural University,Hefei,Anhui 230036)
机构地区:[1]安徽农业大学生命科学学院,安徽合肥230036
出 处:《安徽农业科学》2020年第23期211-213,共3页Journal of Anhui Agricultural Sciences
基 金:安徽省大学生创新创业训练计划项目(201710364099)。
摘 要:[目的]研究洋甘菊挥发油抗炎舒敏、抗氧化及防晒等作用。[方法]水蒸气蒸馏法提取德国洋甘菊挥发油,紫外分光法检测洋甘菊挥发油对DPPH、ABTS自由基的清除作用;Elson-Morgan法测定洋甘菊挥发油对透明质酸酶的抑制作用;胶带法测定洋甘菊挥发油防晒作用。[结果]洋甘菊挥发油得率为0.841%;在研究范围内,挥发油浓度为15 mg/mL时,对透明质酸酶的抑制率达到最大值(61.7%);挥发油浓度为30 mg/mL时,DPPH自由基清除率达到最大值(96.2%);挥发油浓度为150μg/mL时,ABTS自由基清除率达到最大值(94.9%)。胶带法研究挥发油防晒效果,在280~320 nm处吸光度均大于1,有一定的防晒效果。[结论]在一定浓度范围内,洋甘菊挥发油具有抗炎舒敏、抗氧化、防晒等作用。[Objective]To study the effects of Matricaria chamomilla volatile oil on anti-inflammatory,anti-stimulation,anti-oxidation and sunscreen.[Method]The volatile oil of Matricaria chamomilla was extracted by steam distillation,and the scavenging effect of the volatile oil of Matricaria chamomilla on DPPH and ABTS hydroxyl radicals was detected by ultraviolet spectroscopy.The Elson-Morgan method was used to determine the inhibitory effect of Matricaria chamomilla volatile oil on hyaluronidase;the tape method was used to determine the sunscreen effect of Matricaria chamomilla volatile oil.[Result]The yield of chamomile volatile oil was 0.841%.Within the research range,when the concentration of volatile oil was 15 mg/mL,the inhibition rate of hyaluronidase reached the maximum(61.7%).When the volatile oil concentration was 30 mg/mL,the DPPH scavenging rate reached the maximum(96.2%);when the volatile oil concentration was 150μg/mL,the ABTS free radical scavenging rate reached the maximum(94.9%).The tape method was used to study the sunscreen effect of volatile oil,and the absorbance at 280-320 nm was greater than 1,which had a certain sunscreen effect.[Conclusion]Within a certain concentration range,chamomile volatile oil has the effects of anti-inflammatory,anti-stimulation,anti-oxidation and sunscreen.
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