Kiss-1基因过表达对直肠癌SW620细胞增殖的影响  

Expression of Kiss-1 Gene and Its Effect on Proliferation in SW620 Cells of Rectal Cancer

作　　者：方佩斐 陈谨[1] 宋先兵[1] 方永红[1] FANG Pei-fei;CHEN Jin;SONG Xian-bing;FANG Yong-hong(Department of physiology,Anhui Medical College,Anhui Hefei,230061,China)

机构地区：[1]安徽医学高等专科学校生理教研室,安徽合肥230061

出　　处：《承德医学院学报》2020年第6期456-460,共5页Journal of Chengde Medical University

摘　　要：目的探讨Kiss-1基因表达与过表达对直肠癌细胞SW620增殖的影响。方法培养人直肠癌SW620细胞,随机将其分为3组:空白对照组(未转染)、实验组(转染p IRES2-Kiss-1)、阴性对照组(转染p IRES2空载体)。采用免疫荧光染色和免疫细胞化学染色观察直肠癌SW620细胞中Kiss-1的表达情况;Western blot检测转染p IRES2-Kiss-1基因后直肠癌SW620细胞中Kiss-1的表达;采用CCK-8法、MTT法检测转染对SW620细胞增殖的影响。结果免疫重组质粒pIRES2-Kiss-1成功转染至直肠癌SW620细胞,与空白对照组和阴性对照组相比较,实验组中Kiss-1蛋白高表达,差异有显著性(P<0.05);3组细胞培养48、72h后,与空白对照组和阴性对照组SW620细胞数相比较,实验组明显降低(P<0.05)。结论Kiss-1基因在直肠癌SW620细胞中能高效、稳定地表达;Kiss-1基因可有效降低直肠癌SW620细胞的增殖。Objective To investigate the effect of kiss-1 gene expression and overexpression on the proliferation of rectal cancer cells.Methods Human rectal cancer SW620 cells were cultured and randomly divided into 3 groups:blank control group(untransfected),experimental group(transfected with pIRES2-Kiss-1)and negative control group(transfected with empty pIRES2 vector).Immunofluorescence staining and immunocytochemical staining were used to observe the expression of kiss-1 in SW620 cells of rectal cancer.Western blot analysis was employed to examine kiss-1 expression in SW620 cells transfected with piRES2-kiss-1 gene.CCK-8 and MTT assays were used to detect the effect of transfection on SW620 cell proliferation.Results The immune recombinant plasmid pIRES2-Kiss-1 was successfully transfected into rectal cancer SW620 cells.Compared with the blank control group and the negative control group,the kiss-1 protein was highly expressed in the experimental group,and the difference was significant(P<0.05).After cell culture for 48 h and 72 h,the numbers of SW620 cells in the three groups were significantly lower than that in the blank control group and the negative control group(P<0.05).Conclusion Kiss-1 gene can be expressed efficiently and stably in SW620 cells of rectal cancer,which can effectively reduce the proliferation of SW620 cells in rectal cancer.

关 键 词：直肠癌SW620细胞 KISS-1基因 细胞增殖 

分 类 号：R34[医药卫生—基础医学]