甲基环丙稀对干旱胁迫苗期甘蔗叶绿素荧光参数的影响  

Effects of 1-MCP on Chlorophyll Fluorescence Parameters in Sugarcane Seedling Stage Under Drought Stress

作　　者：何姗珊[1] 曾媛[2] 梁朝旭[1] 经艳[1] 唐仕云[1] 李麒鹏 李江平 宁夏[2] 谭芳[1] 李松[1] 李鸣[1] HE Shan-shan;ZENG Yuan;LIANG Zhao-xu;JING Yan;TANG Shi-yun;LI Qi-peng;LI Jiang-ping;NING Xia;TAN Fang;LI Song;LI Ming(Sugarcane Research Institute,Guangxi Academy of Agricultural Sciences,Guangxi Nanning 530007,China;Guangxi Academy of Agricultural Sciences,Guangxi Nanning 530007,China;Dayu Irrigation Group Company Limited,Gansu Jiuquan 735000,China)

机构地区：[1]广西农业科学院甘蔗研究所,广西南宁530007 [2]广西农业科学院,广西南宁530007 [3]大禹节水集团股份有限公司,甘肃酒泉735000

出　　处：《西南农业学报》2020年第10期2198-2204,共7页Southwest China Journal of Agricultural Sciences

基　　金：广西科技攻关重点研发计划项目(桂科AB16380258)。

摘　　要：【目的】探究甲基环丙稀(1-MCP)对干旱胁迫下甘蔗苗期叶绿素荧光参数的影响情况,为苗期甘蔗的干旱生理生化研究提供参考依据。【方法】在封闭空间,对5~8叶苗期甘蔗设置正常供水(处理1)、正常供水+1.0 mg/L 1-MCP熏蒸(处理2)、干旱胁迫(处理3)和干旱胁迫+1.0 mg/L 1-MCP熏蒸(处理4)处理,测定各处理甘蔗叶片的叶绿素含量和叶绿素荧光参数等指标,分析1-MCP对干旱胁迫苗期甘蔗叶绿素荧光参数的影响。【结果】在正常供水时,处理1和处理2甘蔗的叶绿素总含量均保持在较高水平,且相对稳定;在干旱胁迫时,处理3和处理4甘蔗的叶绿素总含量均明显低于处理1和处理2,且随着干旱胁迫时间的延长,处理4甘蔗的叶绿素总含量显著高于处理3(P<0.05,下同)。在正常供水时,处理1和处理2甘蔗叶片的PSII光能转换效率(Fv/Fm)、PSII实际利用量子产量(Yield)、电子传递效率(ETR)、光化学淬灭系数(qP)和叶非光化学淬灭系数(qN)等叶绿素荧光参数较稳定,无显著差异(P>0.05);在干旱胁迫时,处理3和处理4甘蔗叶片的Fv/Fm、Yield、ETR和qP显著低于处理1和处理2,qN显著高于处理1和处理2,其中,在干旱胁迫的第3~5天,处理4甘蔗叶片的Fv/Fm、Yield、ETR和qP均显著高于处理3,qN显著低于处理3。【结论】用1.0 mg/L 1-MCP熏蒸受干旱胁迫的苗期甘蔗,能减缓其叶片叶绿素总含量、Fv/Fm、Yield、ETR和qP的下降速度,降低qN升幅,在短期内有利于缓解干旱对苗期甘蔗的伤害程度,可在苗期甘蔗干旱生理生化研究中参考应用。【Objective】Effects of 1-MCP on chlorophyll fluorescence parameters in sugarcane seedling stage under drought-stress were studied to provide references for physiology and biochemistry research on sugarcane seedlings under drought stress.【Method】In the enclosed space,four treatments were prepared for sugarcane at the seedling stage of 5-8 leaves,which were normal water supply(treatment 1),normal water supply+fumigation 1.0 mg/L 1-MCP(treatment 2),drought stress(treatment 3),and drought stress+fumigation 1.0 mg/L 1-MCP(treatment 4).Chlorophyll content and chlorophyll fluorescence parameters were measured to analyze the effects and working mechanism of 1-MCP on sugarcane seedlings under drought stress.【Result】Under the normal water supply condition,treatment 1and treatment 2 had stable and high chlorophyll content.Under drought stress,treatment 3 and treatment 4 had obviously lower total chlorophyll content than treatment 1 and treatment 2.Along with longer drought stress time,total chlorophyll content in treatment 4 was significantly higher than treatment 3(P<0.05,the same as below).Treatment 1 and treatment 2 had stable chlorophyll fluorescence parameters such as Fv/Fm,Yield,ETR,qP and qN.No significant difference existed between treatment 1and treatment 2(P>0.05).Compared with treatment 1and treatment 2,treatment 3 and treatment 4 under drought stress had significantly lower Fv/Fm,Yield,ETR,and qP while treatment 3 and treatment 4 had significantly higher qN.During 3-5 days,treatment 4 had obviously higher Fv/Fm,Yield,ETR and qP than treatment 3,but treatment 4 had significantly lower qN.【Conclusion】Fumigating sugarcane seedlings with 1.0 mg/L 1-MCP alleviates the degradation rate of total chlorophyll content,Fv/Fm,Yield,ETR and qP and the rise of qN,which can reduce the harm caused by drought on sugarcane for a short period of time.The current study can provide references for physiological and biochemical research of drought-stricken sugarcane at seedling stage.

关 键 词：甘蔗 苗期 干旱胁迫 甲基环丙烯(1-MCP) 叶绿素荧光参数 

分 类 号：S566.1[农业科学—作物学]