基于转录组的三月李及其红肉突变体Expansin基因家族鉴定及分析  

Identification and Analysis of Expansins in the Transcriptome of Sanyueli Plum and Its Red-flesh Mutant

作　　者：方智振[1] 林炎娟[1] 姜翠翠[1] 周丹蓉[1] 潘少霖[1] 叶新福[1] FANG Zhizhen;LIN Yanjuan;JIANG Cuicui;ZHOU Danrong;PAN Shaolin;YE Xinfu(Fruit Research Institute/Fujian Engineering and Technology Research Center for Deciduous Fruit Trees,Fujian Academy of Agricultural Sciences,Fuzhou,Fujian 350013,China)

机构地区：[1]福建省农业科学院果树研究所/福建省落叶果树工程技术研究中心,福建福州350013

出　　处：《福建农业学报》2020年第9期919-928,共10页Fujian Journal of Agricultural Sciences

基　　金：福建省科技计划公益类专项(2018R1013-4、2018R1013-1);福建省自然科学基金项目(30471225);福建省农业科学院青年英才计划项目(YC2015-9);福建省农业科学院创新团队建设项目(STIT2017-1-4)。

摘　　要：【目的】Expansin是广泛存在于植物中的一类细胞壁蛋白,通过调节细胞壁柔韧性在植物生长发育过程中发挥作用。本研究旨在分析Expansin基因家族特征及其在果实成熟过程中的表达模式,并比较Expansin基因在三月李及其红肉突变体之间的差异表达。【方法】基于三月李及其红肉突变体果实成熟过程的转录组数据,采用生物信息学分析方法进行Expansin家族基因鉴定与分析。【结果】鉴定出33个李Expansin基因家族成员,编码蛋白长度在176~460 aa,分子量在19.21~51.33 kD,等电点4.62~9.83,大部分为位于细胞外的稳定亲水蛋白。系统进化树分析表明李Expansin家族成员可分为4个组:22个EXPA、6个EXPB、1个EXPLA和4个EXPLB。所有李Expansin蛋白质均具有保守的DPBB1和Pollenallerg1结构域。保守基序分析表明,同一组Expansin成员具有相似的基序组成。9个Expansin基因在三月李及其红肉突变体果实成熟过程中差异表达。【结论】Expansin在三月李及其红肉突变体果实成熟过程中的表达模式存在显著差异。本研究结果可为解析李Expansin家族基因在果实成熟过程中的功能奠定基础。【Objective】Characteristics and expressions of Expansins,the widely existing gene family associated with plant cell wall,were studied during the fruit ripening stage to decipher the genetic functions relating to ripening of the plum fruits.【Method】Transcriptomes of fruits of Sanyueli(Prunus salicina Lindl.)and its red-flesh mutant were used to identify Expansin genes as well as for a bioinformatic and expression comparison of identified genes.【Result】Thirty-three Expansins,with individual protein containing 176 to 460 amino acids,a molecular weight ranging from 19.21 kD to 51.33 kD,and an isoelectric point varying from 4.62 to 9.83,were identified from the fruit transcriptome data.Most of them were stable hydrophilic proteins located in the extracellular space.Phylogenetic analysis revealed that plum Expansin family included 22 EXPAs,6 EXPBs,1 EXPLA,and 4 EXPLBs.All of the proteins had DPBB1 and Pollenallerg1 domains,and,within a same classification,they shared same conserved motif composition.Nine Expansins were differentially expressed in Sanyueli and the mutant during fruit ripening.The expressions of EVM0015785,EVM0016777,EVM0010710,EVM0022202,EVM0002390,and EVM0024996 significantly differed between Sanyueli and the red-flesh mutant.【Conclusion】The information obtained was to be used for further study on the functions of the Expansin family during ripening of plum fruits.

关 键 词：李 转录组 expansin家族 生物信息学分析 表达分析 

分 类 号：S662.3[农业科学—果树学]