ELISA测定儿童血浆中25-(OH)VitD的临床分析  

Clinical analysis of ELISA for determination of 25-(OH)VitD in children′s plasma

作　　者：李嘉恺 李文胜[2,3] LI Jiakai;LI Wensheng(Legal Affairs Office,West China Second University Hospital,Sichuan University,Chengdu,Sichuan 610041,China;Department of Laboratory Medicine,West China Second University Hospital,Sichuan University,Chengdu,Sichuan 610041,China;Key Laboratory of Birth Defects and Related Diseases of Women and Children,Chengdu,Sichuan 610041,China)

机构地区：[1]四川大学华西第二医院法务部,四川成都610041 [2]四川大学华西第二医院检验科,四川成都610041 [3]出生缺陷与相关妇儿疾病教育部重点实验室,四川成都610041

出　　处：《国际检验医学杂志》2020年第S02期44-46,共3页International Journal of Laboratory Medicine

摘　　要：目的验证酶联免疫吸附法(ELISA)检测儿童血浆中25羟维生素D[25-(OH)VitD]水平的灵敏度和特异度。方法随机选取成都地区0~12岁儿童,共检测150例标本,其中健康儿童126例,佝偻病患儿18例,维生素D缺乏骨畸形患儿6例。采集EDTA-K2抗凝静脉血并分离出血浆,采用ELISA检测血浆25-(OH)VitD的水平。然后结合佝偻病临床诊断,对方法学的灵敏度和特异度进行评价。结果25-(OH)VitD的浓度在6.7~368.0 nmol/L范围内存在线性关系(r=0.8468),方法的回收率为96.78%,临床特异度98.43%,临床灵敏度91.67%。结论本法操作简便准确,临床特异度、灵敏度较高,对人员及仪器要求不高,可以广泛应用于基层医院检验科。Objective To validate the Sensitivity and specificity of ELISA to detect 25-(OH)VitD in children′s plasma.Methods A total of 150 samples were randomly selected from 0-12 years old children in Chengdu,including 126 normal children,18 children with rickets and 6 children with vitamin D deficiency and bone malformation.EDTA-K2 anticoagulant venous blood was collected and the bleeding plasma was separated.The plasma 25-(OH)VitD level was detected by ELISA.Then,combined with the clinical diagnosis of rickets,the sensitivity and specificity of the method were evaluated.Results The concentration of 25-(OH)VitD was linear in the range of 6.7-368.0 nmol/L(r=0.8468).The recoveries were 96.78%,the clinical specificity was 98.43%,and the clinical sensitivity was 91.67%.Conclusion The method is simple,accurate,clinical specificity,high sensitivity,low requirements for personnel and equipment,and can be widely used in basic hospital laboratory.

关 键 词：酶联免疫法 25羟维生素D 佝偻病 

分 类 号：R28[医药卫生—中药学]