3种杉木种子活力测定方法比较  

A comparative study of three determination methods for seed vigor of Cunninghamia lanceolata

作　　者：徐振飞 过晟鹏 钱旺 包苗清 林二培[1] 黄华宏[1] 童再康[1] XU Zhenfei;GUO Shengpeng;QIAN Wang;BAO Miaoqing;LIN Erpei;HUANG Huahong;TONG Zaikang(State Key Laboratory of Subtropical Silviculture,Zhejiang A&F University,Hangzhou 311300,Zhejiang,China)

机构地区：[1]浙江农林大学省部共建亚热带森林培育国家重点实验室,浙江杭州311300

出　　处：《浙江农林大学学报》2020年第6期1230-1234,共5页Journal of Zhejiang A&F University

基　　金：浙江省大学生科技创新活动计划项目(2018R412005)。

摘　　要：【目的】建立一种快速简便的杉木Cunninghamia laneolata种子活力测定方法。【方法】以3个不同品种杉木种子为材料,以标准发芽法为参照,比较四氮唑法(TTC)和紫外分光光度计法(UVS)测定种子活力时的准确性和便捷性。【结果】标准发芽法测定种子活力最为准确,但耗费时间长,难以开展大批量测定;TTC法测定杉木种子活力准确度不稳定,且操作繁琐;UVS法的测定值与真实萌发率比较接近,有较高可信度,且操作简便。【结论】UVS法可作为快速测定杉木种子活力的优选方法。[Objective] This study is aimed to establish a quick and simple method for the determination of the seed vigor of Cunninghamia lanceolata. [Method] With the seeds selected from three different cultivars of C.lanceolata, a comparison was conducted of the accuracy and efficiency of triphenyltetrazolium chloride(TTC)and ultraviolet spectrophotometer(UVS) methods with the standard germination test for reference. [Result]The standard germination test was the most accurate but most time-consuming method, which could not be performed on a large scale. TTC method was unstable and inaccurate with cumbersome operation for C.lanceolata seeds. UVS method displayed an estimated value quite close to the real germination rate and enjoyed advantages such as high reliability and simple operation. [Conclusion] UVS method could be taken as an efficient method for the rapid determination of seed vigor in C. lanceolata.

关 键 词：森林培育学 杉木 种子活力 标准发芽法 紫外分光光度计法(UVS) 四氮唑法(TTC) 

分 类 号：S762.3[农业科学—森林保护学]