基于mTOR信号通路探讨玉女煎对GK大鼠胰岛的保护作用  

Protective effect of Yunv Decoction on pancreas islets of GK rats based on m TOR signaling pathway

作　　者：何才姑[1] 钱长晖[1] 黄玉梅[1] 蒋艺燕[1] 郑雪花[1] 高碧珍[1] HE Cai-gu;QIAN Chang-hui;HUANG Yu-mei;JIANG Yi-yan;ZHENG Xue-hua;GAO Bi-zhen(Fujian University of Traditional Chinese Medicine,Fuzhou 350122,China)

机构地区：[1]福建中医药大学,福州350122

出　　处：《中华中医药杂志》2020年第11期5495-5498,共4页China Journal of Traditional Chinese Medicine and Pharmacy

基　　金：国家自然科学基金面上项目(No.81774144,No.81873234);福建省自然科学基金项目(No.2018J01877)。

摘　　要：目的:探讨玉女煎对GK大鼠胰岛的保护作用及其机制。方法:将GK大鼠随机分为二甲双胍组(100mg·kg^-1·d^-1),玉女煎低、中、高剂量(3.13、6.25、12.50g·kg^-1·d^-1)组和模型组,Wistar大鼠为正常组;分别给予相应药物,模型组和正常组给予0.9%氯化钠溶液。测大鼠随机血糖和体质量;检测胰腺自噬基因ATG12及信号分子哺乳动物雷帕霉素靶蛋白(mTOR)的mRNA和蛋白质表达;观察胰岛微细结构变化。结果:与模型组比较,玉女煎中剂量组血糖显著降低(P<0.01),体质量显著升高(P<0.01),ATG12的mRNA和蛋白质表达显著降低(P<0.05,P<0.01),而mTOR表达显著升高(P<0.05,P<0.01),β细胞内自噬体较少。结论:玉女煎降低GK大鼠血糖,改善胰岛微细结构,可能是通过调节自噬实现的。Objective:To investigate the protective effect and mechanism of Yunv Decoction on pancreas islets of GK rats.Methods:GK rats were randomly divided into metformin group(100 mg·kg-1·d-1),Yunv Decoction 3.13,6.25,12.50 g·kg-1·d-1 groups and model group,and Wistar rats were divided to the normal group.Each drug group was given the corresponding drugs,the model group and the normal group were given 0.9%sodium chloride solution.Body weight and blood glucose were measured.The expression of mRNA and protein of the pancreatic autophagic gene ATG12 and signal molecule mTOR were detected.The morphological changes of pancreas islets were observed.Results:After administration with Yunv Decoction 6.25 mg·kg^-1·d^-1,the blood glucose was significantly lower(P<0.01)and body weight was significantly higher(P<0.01),the expression of mRNA and protein of ATG12 were significantly reduced(P<0.05,P<0.01),and mTOR expression was significantly increased(P<0.05,P<0.01).There was less autophagosomes of beta cells when compared with the model group.Conclusion:Yunv Decoction decreases blood glucose and ameliorates the microstructure of islets through regulating autophagy.

关 键 词：玉女煎 ATG12 GK大鼠 胰岛β细胞 微细结构 糖尿病 血糖 自噬 

分 类 号：R285.5[医药卫生—中药学]