糖肾宁含药血清对高糖培养下足细胞上皮间质转分化的作用  

Effects of drug-containing serum of Tangshenning on podocyte EMT in high-glucose environment

作　　者：崔方强 高彦彬[2,3] 王悦芬 姜嫄嫄[1] 刘志强[1] 赵文景 CUI Fang-qiang;GAO Yan-bin;WANG Yue-fen;JIANG Yuan-yuan;LIU Zhi-qiang;ZHAO Wen-jing(Beijing Hospital of Traditional Chinese Medicine,Capital Medical University,Beijing 100010,China;School of Traditional Chinese Medicine,Capital Medical University,Beijing 100069,China;Beijing Key Laboratory of TCM Collateral Disease Theory Research,Beijing 100069,China)

机构地区：[1]首都医科大学附属北京中医医院,北京100010 [2]首都医科大学中医药学院,北京100069 [3]中医络病研究北京市重点实验室,北京100069

出　　处：《中华中医药杂志》2020年第11期5499-5502,共4页China Journal of Traditional Chinese Medicine and Pharmacy

基　　金：国家自然科学基金项目(No.81703989);北京市自然科学基金项目(No.7182069,No.7172096)。

摘　　要：目的:探讨糖肾宁含药血清对于高糖刺激下足细胞上皮间质转分化的影响。方法:将条件永生小鼠足细胞系分为正常对照组,高糖组,糖肾宁含药血清低、中、高剂量组,CCK-8法检测各组足细胞细胞活力,倒置显微镜观察各组足细胞细胞形态,鬼笔环肽-罗丹明染色观察各组足细胞细胞骨架变化,免疫荧光染色及Western blot法检测各组足细胞P-钙粘蛋白(P-cadherin)、成纤维细胞特异蛋白-1(FSP-1)蛋白表达水平。结果:与正常对照组比较,高糖组足细胞细胞活力降低(P<0.05),细胞形态变化及细胞骨架重构,P-cadherin蛋白表达下调(P<0.05),FSP-1蛋白表达上调(P<0.05);与高糖组比较,糖肾宁含药血清组足细胞细胞活力升高(P<0.05),细胞形态变化及细胞骨架重构减轻,P-cadherin蛋白表达上调(P<0.05),FSP-1蛋白表达下调(P<0.05)。结论:糖肾宁含药血清可以减轻高糖刺激引起的足细胞上皮间质转分化。Objective:To observe the effects of drug-containing serum of Tangshenning(TSN)on podocyte EMT in high-glucose environment.Methods:Differentiated mouse podocytes in vitro were divided into following groups:normal control group,high glucose group,low,medium and high dose drug-containing serum of TSN groups.Cell viability was detected by CCK-8 assay.Cell morphology was observed by microscope and rhodamine staining.Immunofluorescence and Western blot were used for detecting protein expression of P-cadherin and FSP-1 in our study.Results:Compared with normal control group,cell viability of high glucose group was significantly decreased(P<0.05),cell morphological changes and cytoskeleton remodeling,P-cadherin protein expression was significantly decreased(P<0.05),FSP-1 protein expression was significantly increased(P<0.05).Compared with high glucose group,cell viability of drug-containing serum of TSN group were significantly increased(P<0.05),the changes of cell morphology and cytoskeleton remodeling were alleviated,P-cadherin protein expression were significantly increased(P<0.05),FSP-1 protein expression were significantly decreased(P<0.05).Conclusion:Drug-containing serum of TSN significantly inhibited podocyte EMT in high-glucose environment.

关 键 词：糖肾宁 糖尿病肾病 足细胞 上皮间质转分化 P-钙粘蛋白 成纤维细胞特异蛋白-1 

分 类 号：R285.5[医药卫生—中药学]