南葶苈子中槲皮素-3-O-β-D-葡萄糖-7-O-β-D-龙胆双糖苷的稳定性考察  

Stability study of quercetin-3-O-β-D-glucose-7-O-β-D-diglycoside in Descurainia sophia seeds

作　　者：王文平[1] 吴慧敏 刘曼婷 许煜晨 尹兴斌[1] 倪健[1] WANG Wen-ping;WU Hui-min;LIU Man-ting;XU Yu-chen;YIN Xing-bin;NI Jian(School of Chinese Materia Medica,Beijing University of Chinese Medicine,Beijing 102488,China)

机构地区：[1]北京中医药大学中药学院,北京102488

出　　处：《中华中医药杂志》2020年第11期5739-5742,共4页China Journal of Traditional Chinese Medicine and Pharmacy

基　　金：北京市科技计划(No.Z171100001717027)。

摘　　要：目的:探究南葶苈子药材中槲皮素-3-O-β-D-葡萄糖-7-O-β-D-龙胆双糖苷(槲皮素双糖苷)的稳定性,为南葶苈子在临床上的合理应用提供借鉴和参考。方法:采用经典恒温法考察了该成分在水溶液中的热稳定性,得出了不同温度下的反应速率方程,推算出了其有效期和半衰期;以lgK(K为反应速率常数)对1/T进行一元线性回归,得到回归方程;考察该成分在不同pH、不同溶剂以及不同浓度条件下的稳定性情况。结果:该成分在80、100℃条件下加热10h后,含量分别降至初始含量的50.1%、5.4%,热降解规律符合零级反应方程。温度为60、80、100℃时的有效期分别为7.71、2.95、2.39h,半衰期分别为58.29、7.54、5.07h。另外,在pH>6条件下,该成分发生大量降解,为碱催化反应。结论:南葶苈子中的槲皮素双糖苷不稳定,在制剂生产过程中应进行相应条件控制。Objective:To explore the stability of quercetin-3-O-β-D-glucose-7-O-β-D-diglycoside,a medical material of Descurainia sophia seeds and provide reference for rational clinical application of Descurainia sophia seeds.Methods:Through the classical thermostatic method,we investigated the thermal stability of Quercetin-3-O-β-D-glucose-7-O-β-Ddiglycoside in aqueous solution,obtained the reaction rate equation at different temperature and calculated its validity and halflife time;Moreover,the linear regression of 1/T with lgK(K is the rate constant)and the regression equation was calculated;In addition,we investigated the stability of this ingredient under different pH values,solvents and concentrations.Results:After heating this component at 80℃and 100℃for 10 h,the content dropped to 50.08%and 5.4%of the original content,respectively,and the thermal degradation rule conformed to the zero-order reaction equation.When the temperature is 60,80,100℃,the validity period is 7.71,2.95,2.39 h,and the half-life is 58.29,7.54,5.07 h.In addition,under the condition of pH>6,a large amount of this component is degraded,which is an alkali-catalyzed reaction;Conclusion:Quercetin-3-O-β-Dglucose-7-O-β-D-gentiobioside in Descurainia sophia seeds is unstable,and corresponding conditions should be controlled during the preparation process.

关 键 词：南葶苈子 槲皮素-3-O-β-D-葡萄糖-7-O-β-D-龙胆双糖苷 热稳定性 PH值 

分 类 号：R284.1[医药卫生—中药学]