基于泛素-蛋白酶体通路研究杜仲提取物治疗动脉粥样硬化的机制  

Mechanism of Eucommiae Cortex extract inhibiting the progress of atherosclerosis based on the ubiquitin-proteasome pathway

作　　者：庞军 吴强[1] 程文立 李玲[1] 刘承铭 刘微 黄晶[1] PANG Jun;WU Qiang;CHENG Wen-li;LI Ling;LIU Cheng-ming;LIU Wei;HUANG Jing(Guizhou Provincial People’s Hospital,Guiyang 550000,China;Department of Hypertension,Beijing Anzhen Hospital,Capital Medical Vniversity,Beijing 100029,China)

机构地区：[1]贵州省人民医院,贵阳550000 [2]首都医科大学附属北京安贞医院高血压科,北京100029

出　　处：《中华中医药杂志》2020年第10期5279-5281,共3页China Journal of Traditional Chinese Medicine and Pharmacy

基　　金：贵州省基础研究计划项目(No.黔科合基础[2019]1190号);国家自然科学基金地区基金项目(No.81760071)。

摘　　要：目的:探讨杜仲提取物对小鼠主动脉粥样硬化(AS)模型中泛素-蛋白酶体通路(UPP)关键因子的影响及机制。方法:取载脂蛋白E基因敲除(APOE-/-)小鼠30只给予高脂饲料喂养,另取C57BL/6小鼠10只以普通饲料饲养为对照组。在确认高脂组AS模型建立后,采用杜仲提取物治疗组灌胃1、2、4个月,分别测小鼠血清中胆固醇(CH)、甘油三酯(TG)、低密度脂蛋白(LDL)的含量;取小鼠胸主动脉切片光镜下观察,通过免疫组织化学方法检测主动脉组织UPP相关蛋白表达。结果:经过杜仲提取物干预后,治疗组2、4月组小鼠血清CH较高脂组显著降低(P<0.05),MMPS、NF-κB、CD54/ICAM-1、p53、CD40L/CD154、p27蛋白表达程度在干预后1、2、4月呈现降低趋势。结论:杜仲提取物能一定程度上降低APOE-/-小鼠血清CH浓度,其途径可能通过抑制UPP相关蛋白表达实现。Objective:To explore the effects and mechanism of Eucommiae Cortex extract on the aortic atherosclerotic plaque through ubiquitin protease pathway.Methods:A total of 30 apolipoprotein E knockout(APOE-/-)mice were fed with highfat diet to establish an atherosclerosis model,and 10 C57 BL/6 mice were fed with normal diet as control group.After 4 months,the blood lipids were randomly checked to confirm the establishment of the atherosclerosis model.Eucommiae Cortex extracts were gavaged for 1,2,and 4 months,respectively.The content of cholesterol,triglyceride and low-density lipoprotein were detected.The thoracic aorta of mice was taken to observe arterial plaque under light microscope.The expression of ubiquitinprotease pathway related proteins in aorta tissue was detected by immunohistochemistry.Results:After the intervention of Eucommiae Cortex extract,the serum cholesterol of mice decreased(P<0.05),and the protein expression levels of MMPS,NF-κB,CD54/ICAM-1,p53,CD40 L/CD154,and p27 decreased in 1,2,and 4 months after the intervention.Conclusion:Eucommiae Cortex extract can reduce the serum cholesterol concentration of APOE-/-mice to a certain extent,and its pathway may be the inhibition the expression of ubiquitin-proteasome pathway related protein.

关 键 词：杜仲提取物 泛素-蛋白酶体通路 载脂蛋白E基因敲除小鼠 动脉粥样硬化 机制 绿原酸 

分 类 号：R285.5[医药卫生—中药学]