鼠疫耶尔森菌选择培养基的筛选试验  

Screening test of Yersinia pestis selective medium

作　　者：谢辉[1] 祁芝珍[1] 李君[1] 赵小龙[1] 王梅[1] 张爱萍[1] 任玲玲[1] XIE Hui;QI Zhizheng;LI Jun;ZHAO Xiaolong;WANG Mei;ZHANG Aiping;REN Lingling(Jinghai Province Institute for Endemic Disease Prevention and Control,Xining 811602,China)

机构地区：[1]青海省地方病预防控制所,西宁811602

出　　处：《医学动物防制》2020年第12期1168-1170,共3页Journal of Medical Pest Control

基　　金：国家卫生健康委员会鼠疫防治研究重点实验室(青海省地方病预防控制所)(2019PT310004);青海省鼠疫防控及研究重点实验项目(2020-ZJ-Y23)。

摘　　要：目的研究多种鼠疫耶尔森菌选择培养基进行比对验证,筛选出一种可以有效抑制部分常见致病菌以提高分离鼠疫菌效率的选择培养基。方法将5种实验菌株分别接种在改良胆碲铜紫选择溶血培养基、5%大蒜汁选择培养基、0.1%水合氯醛选择培养基、青霉素选择培养基和庆大霉素选择培养基进行鼠疫菌分离培养。结果5种实验菌株在5%大蒜汁选择培养基、青霉素选择培养基和庆大霉素选择培养基生长良好,改良胆碲铜紫选择溶血培养基上鼠疫耶尔森菌、假结核耶尔森菌、小肠结肠炎耶尔森菌生长良好,其中鼠疫耶尔森菌菌落形态典型,其它试验菌株未生长,变形杆菌在0.1%水合氯醛选择培养基上受抑制未生长。结论改良胆碲铜紫选择溶血培养基是较为理想的选择培养基,0.1%水合氯醛选择培养基可作为抑制变形杆菌的选择培养基。Objective To study multiple Yersinia pestis selective mediums through comparison,to screen out a selective medium that can effectively inhibit common pathogenic bacteria in order to improve the efficiency of isolating Yersinia pestis.Methods Totally 5 kinds of experimental strains were respectively cultured on modified bile salt selective hemolysis medium containing tellurium ion and cuprum ion,5%garlic juice selective medium,0.1%chloral hydrate selective medium,penicillin selective medium and gentamicin selective medium for the isolation culture of Yersinia pestis.Results The 5 kinds of experimental strains were cultured well on 5%garlic juice selective medium,penicillin selective medium and gentamicin selective medium.On modified bile salt selective hemolysis medium containing tellurium ion and cuprum ion,Yersinia pestis,Yersinia pseudotuberculosis and Yersinia enterocolitica were cultured well.And the colonial morphology of Yersinia pestis was typical.There was no growth observed in other experimental strains and Proteus vulgaris were restrained from growing in 0.1%chloral hydrate selective medium.Conclusion The modified bile salt selective hemolysis medium containing tellurium ion and cuprum ion is a relatively ideal selective medium,and the 0.1%chloral hydrate selective medium can serve as a selective medium that can inhibit Proteus vulgaris.

关 键 词：鼠疫耶尔森菌 选择培养基 分离培养 筛选 

分 类 号：K378.61[历史地理—历史学]