不同部位腧穴配伍电针对肥胖大鼠胃肠运动及相关激素的影响  

Electroacupuncture improves obesity by lowering gastrointestinal motility, blood lipids and expression of intestinal leptin and cholecystokinin in obese rats

作　　者：王雅媛 梁凤霞 陈丽 卢威 周钰点 黄湘茜 杨姝瑞 WANG Ya-yuan;LIANG Feng-xia;CHEN Li;LU Wei;ZHOU Yu-dian;HUANG Xiang-xi;YANG Shu-rui(College of Acupuncture-moxibustion and Orthopedics-traumatology,Hubei University of Chinese Medicine,Wuhan 430061,China)

机构地区：[1]湖北中医药大学针灸骨伤学院/针灸治未病湖北省协同创新中心,武汉430061

出　　处：《针刺研究》2020年第11期875-881,共7页Acupuncture Research

基　　金：国家自然科学基金面上项目(No.81774420);国家自然科学基金青年基金项目(No.81804170);湖北中医药大学2019年度重大项目培育计划。

摘　　要：目的:观察不同部位腧穴配伍电针对肥胖大鼠胃肠运动及瘦素(LEP)、胆囊收缩素(CCK)的影响,探讨针刺减肥的相关机制。方法:从70只Wistar雄性大鼠中随机挑选10只为正常组,予以普通饲料饲养,其他大鼠采用高脂饮食饲养法造模,共8周。造模成功的40只大鼠随机分为模型组、腹部电针组、下肢部电针组及标本电针组,每组各10只。腹部电针组取"关元""中脘""天枢";下肢部电针组取"足三里""丰隆";标本电针组取腹部联合下肢部穴位,3组均电针20 min/次,3次/周,连续干预8周。观察并记录各组大鼠进食量、体质量、白色脂肪含量;检测大鼠胃排空率和小肠推进率;以全自动生化分析仪检测大鼠血清总胆固醇(TC)、甘油三酯(TG)、游离脂肪酸(NEFA);用ELISA法检测大鼠血清LEP、CCK的含量;用Wes-tern blot法检测小肠LEP、CCK的蛋白表达。结果:干预前与正常组比较,模型组及3个干预组进食量、体质量均升高(P<0.05)。干预后与正常组比较,模型组进食量、体质量、TC、TG、NEFA、白色脂肪含量、胃排空率、血清及小肠的LEP含量显著升高(P<0.01),小肠推进率、血清及小肠CCK含量显著降低(P<0.01)。干预后与模型组比较,3个干预组进食量、体质量、TC、TG、白色脂肪含量、胃排空率、血清及小肠的LEP含量均显著降低(P<0.05,P<0.01),小肠推进率、血清及小肠CCK含量显著升高(P<0.05,P<0.01),下肢部电针组、标本电针组血清NEFA含量显著下降(P<0.01)。与腹部电针组比较,下肢部电针组及标本电针组血清NEFA显著降低(P<0.05)。结论:针刺腹部、下肢部穴位对胃肠运动和LEP、CCK的调节作用一致,但在调节血清NEFA含量上存在一定差异。针刺腹部、下肢部穴位均可改善大鼠的肥胖状态,其机制可能与调控LEP、CCK的表达,从而延迟胃排空,加快小肠推进率,减少进食量有关。Objective To investigate the effect of electroacupuncture(EA)on gastrointestinal motility and expression of leptin(LEP)and cholecystokinin(CCK)in the small intestine in obese rats,so as to explore the mechanism of EA underlying improvement of obesity.Methods Male Wistar rats were randomized into 5 groups:normal control,obesity model,abdominal acupoints["Guanyuan"(CV4),"Zhongwan"(CV12)and bilateral"Tianshu"(ST25)],lower-leg acupoints[bilateral"Zusanli"(ST36)and bilateral"Fenglong"(ST40)],and abdominal+lower-leg acupoints(n=10 rats in each group).The obesity model was established by feeding the animals with high-fat diet for 8 weeks.EA was applied to the abovementioned acupoints for 20 min every time,3 times a week for 8 weeks.The food intake and body mass were recorded.The white adipose tissue around the testicles and in the abdominal region was weighed.The serum total cholesterol(TC),triglyceride(TG)and non-esterified fatty acid(NEFA)were detected by using automatic biochemical analyzer.The gastric empty rate and intestinal propulsive rate were calculated.The contents of serum CCK and LEP were detected by using ELISA,and the expression levels of CCK and LEP proteins in the small intestine were detected by using Western blot.Results Following modeling,the food intake,body mass,weight of white adipose around the testicles and abdomen,the gastric empty rate,and serum TC,TG,NEFA and LEP contents as well as intestinal LEP expression were significantly increased(P<0.05,P<0.01),while the intestinal propulsive rate,serum CCK content and intestinal CCK expression were evidently decreased(P<0.01)in the model group relevant to the normal control group.After EA interventions and compared with the model group,the increased levels of food intake,body mass,white adipose weight,gastric empty rate,TC,TG,and LEP in serum and small intestine,and the decreased levels of intestinal propulsive rate,CCK in the serum and intestine were reversed in the abdominal acupoints,lower-leg acupoints and abdominal+lower-leg acupoints groups(P<0.0

关 键 词：腧穴配伍 电针 肥胖 胃肠运动 胆囊收缩素 瘦素 

分 类 号：R245.9+7[医药卫生—针灸推拿学]