德氏乳杆菌保加利亚亚种发酵产物对常见病原菌的抑制作用  

Inhibitory effect of fermentation products of Lactobacillus delbrueckii subsp.bulgaricus on common pathogens

作　　者：陈华 彭浩[2] 胡方兴 梁伟 张镓 魏莎 邬国军 CHEN Hua;PENG Hao;HU Fangxing;LIANG Wei;ZHANG Jia;WEI Sha;WU Guojun(Department of Clinical Laborboratory,the Third Hospital of Changsha,Changsha,Hunan 410015,China;Department of Burns and Plastic Surgery,the Third Xiangya Hospital of Central South University,Changsha,Hunan 410006,China;Department of Microbiology,School of Basic Medical Sciences,Central South University,Changsha,Hunan 410078,China)

机构地区：[1]湖南省长沙市第三医院检验科,湖南长沙410015 [2]中南大学湘雅三医院烧伤整形外科,湖南长沙410006 [3]中南大学基础医学院微生物学系,湖南长沙410078

出　　处：《国际检验医学杂志》2020年第23期2898-2903,2907,共7页International Journal of Laboratory Medicine

摘　　要：目的探究德氏乳杆菌保加利亚亚种的发酵产物对常见病原菌的抑制作用。方法采用细菌共培养法及牛津杯药物敏感试验,分别探究德氏乳杆菌保加利亚亚种及其发酵产物对11种常见病原菌的抑制作用,并通过乙醇沉淀、透析、超滤离心及饱和硫酸铵盐析法,对德氏乳杆菌保加利亚亚种发酵产物中抑菌物成分进行了初步研究。结果德氏乳杆菌保加利亚亚种发酵产物对多种临床常见病原菌均有较强的抑菌活性,抑菌活性与该菌代谢产生的乳酸无关,且该抑菌活性具有良好的热稳定性;发酵产物的乙醇沉淀物及80%饱和硫酸铵盐析沉淀物均无抑菌活性,以相对分子质量3×103及10×103超滤离心管将发酵液进行超滤离心后进行抑菌试验,显示滤出液及上层液均具有良好的抑菌活性,发酵产物经截留相对分子质量为0.5×103的透析袋透析后失去抑菌活性。结论德氏乳杆菌保加利亚亚种发酵产物对于临床常见的多种革兰阳性、革兰阴性病原菌均具有较强的抑菌活性,其抑菌活性物质可能是相对分子质量0.5×103左右或以下的小分子物质。Objective To explore the inhibitory effects of fermentation products of Lactobacillus delbrueckii subsp.bulgaricus on common pathogens.Methods The inhibitory effect of Lactobacillus delbrueckii subsp.bulgaricus and its fermentation products on 11 common pathogens were investigated by bacterial co-cultivation method and Oxford cup drug sensitivity test.The active ingredients of inhibitory effect in the fermentation products of Lactobacillus delbrueckii subsp.bulgaricus were studied by way of ethanol precipitation,dialysis,ultrafiltration centrifugation and salting out with ammonium sulfate.Results The fermentation products of Lactobacillus delbrueckii subsp.Bulgaricus had strong antibacterial activity against a variety of clinical common pathogens.The antibacterial activity was not related to the lactic acid produced by the strain,and the antibacterial activity had good thermal stability.The ethanol precipitate and 80%saturated ammonium sulfate precipitate of fermentation products had no antibacterial activity.The fermentation products was ultrafiltrated and centrifuged with a relative molecular weight of 3×103 and 10×103 ultrafiltration centrifuge tubes.The results showed that both the filtrate and the upper liquid had good antibacterial activity.The fermentation broth lost the antibacterial activity after dialyzing the retention phase to the dialysis bag with molecular weight of 0.5×103.Conclusion The fermentation product of Lactobacillus delbrueckii subsp.bulgaricus has strong bacteriostatic activity against a variety of clinically common gram-positive and gram-negative pathogens,and its bacteriostatic active substance may be small molecular substances with relative molecular weight of 0.5×103 or less.

关 键 词：微生物敏感试验 德氏乳杆菌 抗药性 细菌 

分 类 号：R446.5[医药卫生—诊断学]