基于病毒宏基因组技术的浮游病毒多样性研究进展  

Research on Diversity of Virioplankton Based on Viral Metagenomics

作　　者：李樾 袁智 刘丽[2] Li Yue;Yuan Zhi;Liu Li(College of Technology,Hubei Engineering University,Xiaogan,Hubei 432000,China;Faculty of Life Science and Technology,Kunming University of Science and Technology,Kunming,Yunnan 650500,China)

机构地区：[1]湖北工程学院新技术学院,湖北孝感432000 [2]昆明理工大学生命科学与技术学院,云南昆明650500

出　　处：《湖北工程学院学报》2020年第6期42-48,共7页Journal of Hubei Engineering University

基　　金：国家自然科学基金(31860028);湖北省教育厅科学研究项目(B2017504);湖北工程学院新技术学院科学研究项目(2019Hgxky06,2017Hgxky19)。

摘　　要：浮游病毒通过调节微生物群落结构与功能来影响水生生态系统的演变,对浮游病毒的全面了解有利于水体污染的防治和生态环境的保持。因依赖于宿主培养的传统病毒研究中存在许多不可避免的局限性,导致对于浮游病毒多样性了解比较片面。病毒宏基因组技术的出现突破了病毒无通用分子靶基因的瓶颈,为研究已知及未知病毒与不同宿主、环境的相关性提供了有力支持。本文总结并讨论了浮游病毒多样性的研究方法及优缺点,综述了病毒宏基因组技术在浮游病毒多样性研究中的研究进展,同时也提出了病毒宏基因组研究中存在的一些问题和展望。Virioplankton affect the evolution of aquatic ecosystems by regulating the structure and function of microbial communities.A comprehensive understanding of virioplankton is beneficial to the prevention and control of water pollution and the maintenance of ecological environment.Due to many inevitable limitations in traditional virus studies that rely on host culture,the understanding of virioplankton diversity is relatively limited.Viral metagenomics technology breaks the bottleneck of the lack of common molecular markers in virus and provides strong support for the study on the correlation between known and unknown viruses and different hosts and environments.In this article,the authors summarized and discussed the research methods,advantages and disadvantages of virioplankton diversity,and reviewed the recent advances in researches on viralmetagenomics in the study of virioplankton diversity.In addition,some tips for problems in and prospects of the researches in this field were also discussed.

关 键 词：病毒宏基因组学 浮游病毒 遗传多样性 

分 类 号：Q933[生物学—微生物学] X524[环境科学与工程—环境工程]