电离辐射对小鼠骨髓造血干/祖细胞中CD47表达的影响  

Effect of ionizing irradiation on expression of CD47 in bone marrow hematopoietic stem/progenitor cells in mice

作　　者：谢光耀 王锋[1] 张婷[1] 刘岩厚[1] 杨永广[1] XIE Guangyao;WANG Feng;ZHANG Ting;LIU Yanhou;YANG Yongguang(Academy of Translational Medicine,National-Local Joint Engineering Laboratory of Animal Models for Human Diseases,First Hospital,Jilin University,Changchun 130021,China)

机构地区：[1]吉林大学第一医院转化医学研究院人类疾病动物模型国家地方联合工程实验室,吉林长春130062

出　　处：《吉林大学学报（医学版）》2020年第6期1169-1176,I0004,共9页Journal of Jilin University：Medicine Edition

基　　金：国家自然科学基金委重大研究计划重点项目资助课题(91642208)。

摘　　要：目的:探讨X射线全身照射后小鼠骨髓造血干/祖细胞(HSCs/HPCs)损伤和其中CD47表达水平的变化,初步阐明CD47在HSCs/HPCs电离辐射损伤中发挥的作用。方法:将C57BL/6小鼠分为未照射组和照射组,其中照射组分为照射后18 h组、5 d组、11 d组和6周组,每组5只。除未照射组小鼠外,其他小鼠均一次性给予3.0或9.0 Gy X射线全身照射。在照射后相应时间点处死小鼠,流式细胞术检测各组小鼠骨髓HSCs与HPCs的百分率。检测照射后各时间点各组小鼠HSCs和HPCs中CD47表达水平。结果:未照射组小鼠HSCs中CD47表达水平高于骨髓成熟造血系细胞1倍以上(P<0.01)。X射线照射后18 h、5 d与11 d后,与未照射组比较,照射组小鼠HSCs中CD47表达水平差异无统计学意义(P>0.05);HPCs占lineage-negative(lin-)细胞百分率或占全骨髓细胞百分率降低(P<0.05),照射后6周HSCs百分率恢复至正常水平。与未照射组比较,照射后18 h HSCs中CD47表达水平差异明显升高(P<0.01),照射后5 d、11 d和6周组小鼠HSCs中CD47表达水平差异无统计学意义(P>0.05);照射后18 h组小鼠HSCs占lin-细胞百分率升高(P<0.01),照射后11 d组小鼠HSCs占全骨髓细胞百分率降低(P<0.01)。照射后18 h组小鼠HPCs中CD47低表达亚群的百分率明显低于CD47高表达亚群(P<0.01)。照射后5 d组、照射后11 d组和照射组后6周组小鼠HPCs中CD47低表达亚群的百分率与CD47高表达亚群的百分率相近。结论:全身电离辐射造成了小鼠骨髓组织损伤,在骨髓细胞中HSCs/HPCs百分率降低,其机制可能与照射后HSCs中CD47表达一过性升高有关联。Objective:To investigate the damage of bone marrow hematopoietic stem/progenitor cells(HSCs/HPCs)and the changes of CD47 expression levels in the HSCs/HPCs of the mice after X-ray irradiation,and to clarify the role of CD47 in irradiation-induced injury of HSCs/HPCs.Methods:The C57BL/6 mice were divided into nonirradation group and irradation group,and the C57BL/6 mice in irradation group were divided into 18 h,5 d,11 d,and 6 weeks after irradiation subgroups,and there were 5 mice in each group.The mice in irradation groups were given by an X-ray irradiator with a dose of 3.0 or 9.0 Gy at one time.The mice were sacrificed at different time points after irradiation.The percentages of HSCs to HPCs in bone marrow of the mice in various groups were detected by flow cytometry,and the expression levels of CD47 in HSCs/HPCs of the mice in various groups were detected at different time points after irradiation.Results:The level of CD47 expression in HPCs of the mice in non-irradation group was more than 2-fold higher than the mature HSCs in bone marrow(P<0.05).Compared with non-irradation group,there were no statistical differences in the CD47 expression levels in HPCs of the mice in irradation groups at 18 h,5 d,and 11 d after irradation(P>0.05);the percentages of HPCs in lineage-negative(lin-)cells and whole bone marrow cells were decreased significantly(P<0.05),which returned to normal level at 6 weeks after irradiation.Compared with non-irradiation group,the CD47 expression level in HSCs of the mice in 18 h after irradiation group was increased(P<0.01),and there were no significant differences in the CD47 expression levels in HSCs of the mice in 5 d,11 d and 6 weeks after irradiation groups(P>0.05);the percentage of HSCs in Lin-cells in 18 h after irradiation group was increased(P<0.01),and the percentage of HSCs cells in whole bone marrow cells in 11 d after irradiation group was decreased(P<0.01).The percentage of CD47 low expression subgroup was significantly lower than that of CD47 high expression subgroup in the

关 键 词：电离辐射 骨髓抑制 造血干细胞 造血祖细胞 CD47 

分 类 号：R144.1[医药卫生—公共卫生与预防医学] R81