C-型利钠肽对肉鸡骨骼肌发育的调控作用研究  

Study on C-type Natriuretic Peptide Regulation of Skeletal Muscle Development in Broiler

作　　者：黄华云[1] 梁忠[1] 刘龙洲[1,2] 赵振华 张欣[1,3] 王钱保 李春苗[1] 黄正洋[1] 黎寿丰[1] HUANG Huayun;LIANG Zhong;LIU Longzhou;ZHAO Zhenhua;ZHANG Xin;WANG Qianbao;LI Chunmiao;HUANG Zhengyang;LI Shoufeng(Jiangsu Institute Poultry Science,Yangzhou,Jiangsu 225125;Yangtze University,Jinzhou,Hubei 434023;Yangzhou University,Yangzhou,Jiangsu 225009)

机构地区：[1]江苏省家禽科学研究所,江苏扬州225125 [2]长江大学,湖北荆州434023 [3]扬州大学,江苏扬州225009

出　　处：《中国家禽》2020年第11期17-21,共5页China Poultry

基　　金：江苏省自然科学基金(BK20191217);现代农业产业技术体系建设专项(CARS-41-Z05);江苏省成果转化项目(BA2019049);省属公益类科研院所自主科研经费项目(BM2018026-2);扬州市科技计划项目(YZ2018045);江苏省农业科技自主创新资金项目(CX(20)2012);扬州市现代农业项目(YZ2019040)。

摘　　要：通过qPCR技术检测NPRB和NPRC在矮小品系(S3系)和隐性白羽肉鸡(RR)胸肌组织中的表达模式,结合C-型利钠肽(CNP)激素对成肌细胞分化,脂滴沉积和分解的作用,阐明CNP对肉鸡肌肉发育的调控作用。结果显示:在19日胚龄、4.8和16周,NPRB和NPRC mRNA在S3系鸡胸肌组织中的表达显著高于RR鸡(P<0.05);CNP外源诱导鸡的原代成肌细胞6 d后,与对照组相较,成肌细胞分化标志基因(MYOG和MYOD)的表达显著升高(P<0.01),但成肌细胞的脂滴沉积能力显著降低(P<0.05)、培养基中甘油浓度显著升高(P<0.05)。以上结果提示,CNP通过促进成肌细胞的分化,抑制脂滴沉积,促进脂分解的作用,进而调控骨骼肌的发育。In this study, the expression pattern of NPRB(CNP receptor) and NPRC(clearance receptor) in the pectinal tissues was detected in S3(sex-linked dwarf chicken) and RR(recessive White Rock chickens) chickens using qPCR. Combined with the effects of CNP on myoblast differentiation, lipid droplet deposition and decomposition, the regulatory function CNP on skeletal muscle development was elucidated. The results showed that the expression of NPRB and NPRC mRNA in the breast muscle of S3 strain chickens was significantly higher than that of RR chickens(P<0.05) at developmental stages of E19, 4 W, 8 W and 16 W;Expression of MyoG and MyoD(marker genes for myoblasts differentiation) was significantly higher than that of the control group(P<0.01) after 6 days of CNP induction. However,the ability of lipid droplet deposition of myoblasts decreased significantly(P<0.05), and the glycerolconcentration in the medium increased significantly(P<0.05). These results suggested that CNP regulates the development of skeletal muscle through up-regulating the differentiation of myoblasts, inhibiting the lipid drop deposition of myoblasts and promoting lipid decomposition.

关 键 词：C-型利钠肽 成肌细胞 脂肪沉积 肉鸡 

分 类 号：S831.2[农业科学—畜牧学]