人参皂苷Rg1对CD133^+和CD34^+脐血造血干细胞增殖分化的影响  

Effects Comparison of Ginsenoside Rg1 on Proliferation and Differentiation of CD133^+ and CD34^+ Umbilical Cord Blood Hematopoietic Stem Cells

作　　者：徐尚福 潘丽 袁毅 陈艳 范振海 方宁 余丽梅 XU Shangfu;PAN Li;YUAN Yi;CHEN Yan;FAN Zhenhai;FANG Ning;YU Limei(The Key Laboratory of Cell Engineering of Guizhou Province of The Affiliated Hospital of Zunyi Medical University,Zunyi 563000,Guizhou,China;The Key Laboratory for Basic Pharmacology of Ministry,of Education,Zunyi Medical University,Zunyi 563000,Guizhou,China;Department of Anatomy,Zunyi Medical University,Zunyi 563000,Guizhou,China;Department of Phamiacy,Guizhou Provincal Peoples Hospital,Guiyang 550001,Guizhou,China;Department of Pediatrics of The Affiliated Hospital of ZunYi Medical University,Zunyi 563000,Guizhou,China)

机构地区：[1]遵义医科大学附属医院贵州省细胞工程重点实验室,贵州遵义563000 [2]遵义医科大学基础药理教育部重点实验室,贵州遵义563000 [3]遵义医科大学解剖学教研室,贵州遵义563000 [4]贵州省人民医院药剂科,贵州贵阳550001 [5]遵义医科大学附属医院儿科,贵州遵义563000

出　　处：《中华中医药学刊》2020年第11期37-40,I0014,I0015,I0016,共7页Chinese Archives of Traditional Chinese Medicine

基　　金：国家自然科学基金(81260307,81560592);贵州省科学技术基金(黔科合J字[2011]2318);遵义医科大学硕士启动基金(201008)。

摘　　要：目的观察人参皂苷Rg1(ginsenosides Rg1, Rg1)对CD133^+和CD34^+人脐血造血干细胞(human umbilical cord blood hemopoietic stem cells, hUCB-HSCs)增殖、分化的影响。方法密度梯度离心及免疫磁珠法,富集、分选CD133^+和CD34^+ hUCB-HSCs,流式细胞术检测分选细胞纯度,0.1、0.3和3μmol/L的Rg1处理培养的hUCB-HSCs 14 d,显微镜下观察细胞形态,计数集落和细胞数。结果免疫磁珠分选所得CD133^+、CD34^+hUCB-HSCs的纯度分别为>90%和>80%,3个浓度的Rg1均不同程度的增加细胞数量,Rg1可促进CD133^+和CD34^+ hUCB-HSCs细胞增殖,增加爆式红系集落形成单位、粒-单集落形成单位集落生成(P<0.05),Rg1对两种HSCs增殖的数量及向红系细胞分化的影响相似,但使CD34^+hUCB-HSCs向粒系和单核系细胞分化的作用明显强于CD133^+hUCB-HSCs。结论 Rg1促进CD133^+和CD34^+ hUCB-HSCs增殖及向红系细胞分化的作用相当,但促CD34^+ hUCB-HSCs向粒单系细胞分化的作用更强。Objective To observe the effect of ginsenosides Rg1(Rg1) on the proliferation and differentiation of human umbilical cord blood hemopoietic stem cells(hUCB-HSCs). Methods Density gradient centrifugation and magnetic cell sorting assay were simultaneously used to enrich and separate the hUCB-HSCs. Cells purity was detected by flow cytometry. The hUCB-HSCs were cultured with treatment by Rg1(0.1, 0.3,3 μmol/L) for 14 days. The cell morphology was observed under inverted phase contrast microscope. The cell numbers and colony forming were counted. Results The percentages of CD34^+ and CD133^+ hUCB-HSCs were more than 90% and 80% after magnetic cells sorting. The cells numbers were obviously increased by three concentrations of Rg1. And Rg1 also could promote the colony formation of burst-forming unit erythroid(BFU-E) and colony forming unit-granulocyte macrophage(CFU-GM) in CD34^+ and CD133^+ hUCB-HSCs. However, the differentiation effect of CD34^+ into granular and monocytes was significantly higher than that of CD133^+ hUCB-HSCs. Conclusion Rg1 affects proliferation and differentiation of CD133^+ and CD34^+ hUCB-HSCs into erythrocytes equivalently, but promotes the differentiation of CD34^+ hUCB-HSCs into granulocytes in a stronger way.

关 键 词：人参皂苷RG1 人脐带血 造血干细胞 增殖 分化 

分 类 号：R285.5[医药卫生—中药学]