大黄的炮制及不同炮制品番泻苷A和番泻苷B含量测定方法的建立  

Processing of Dahuang(Rhubarb) and Establishment of Determination Methods of Sennanoside A and Sennanoside B in Different Processed Products

作　　者：曾超[1] 陆梅元 莫婷婷 覃艳淑 黄敏[1] ZENG Chao;LU Meiyuan;MO Tingting;QIN Yanshu;HUANG Min(The First Affiliated Hospital of Guangxi University of Chinese Medicine,Nanning 530023,Guangxi,China;Guangxi District Food and Drug Review and Inspection Center,Nanning 530029,Guangxi,China;Guangxi-ASEAN Center FOR food and Drug Safety Control,Nanning 530001,Guangxi,China)

机构地区：[1]广西中医药大学第一附属医院,广西南宁530023 [2]广西壮族自治区食品药品审评查验中心,广西南宁530029 [3]广西东盟食品药品安全检验检测中心,广西南宁530001

出　　处：《中华中医药学刊》2020年第11期47-52,I0017,共7页Chinese Archives of Traditional Chinese Medicine

基　　金：国家自然科学基金(81360524);全国中药特色技术传承人才培训项目(2015481601003);国家中医药管理局科研三级实验室中(壮)药化学与质量分析实验室(国中医药发2009[21]号);广西高校中青年教师科研基础能力提升项目(2019KY0341);广西中医药大学青年基金项目(2019QN036)。

摘　　要：目的建立反相高效液相色谱法测定大黄不同炮制品中番泻苷A和番泻苷B的含量。比较其含量差异,揭示中药炮制前后物质基础的变化规律。方法建立采用HPLC法,色谱柱为Inertsil 0DS-3 C18柱(4.60 mm×250 mm, 5μm),流动相∶甲醇-0.1%磷酸(28∶72),检测波长329 nm,流速1.0 mL/min,柱温30℃,进样量10μL,梯度洗脱。结果反相高效液相色谱法测定番泻苷A和番泻苷B,线性方程为:番泻苷A:Y=836.27X-12.551,番泻苷B:Y=668.74X-12.965,分别在0.120 5-1.205(R^2=0.999 7)、0.116-1.160(R^2=0.999 1)的线性范围内呈良好线性关系,大黄不同炮制品中番泻背A和番泻背B的含量差异显著,或增或减,说明炮制工艺对中药大黄各成分影响不同。结论:建立了高效液相色谱法测定大黄不同炮制品中番泻苷A和番泻苷B的含量,该方法可作为大黄及其不同炮制品的质量控制研究的方法。Objective The author determined the contents of sennoside A and sennoside B in different processed products of Dahuang(rhubarb) through establishing a reverse-phase high performance liquid chromatography(HPLC) method. And then the author revealed the material basis of the change law of the crude and processed Chinese medicine,comparing their difference of contents. Methods An HPLC method was established. The chromatographic column was Inertsil ODS-3 C18 column(4.60 mm×250 mm, 5 μm) and the flow phase was methanol-0.1% phosphoric acid(28∶72). The detector was 329 nm and the velocity was 1.0 mL/min. The column temperature was 30 ℃ and the sampling quantity was 10 μL. Results HPLC method was used to determine sennoside A and sennoside B, and the linear equation was as follows: A: Y=836.27 X-12.551;B: Y=668.74 X-12.965, respectively at 0.120 5-1.205(R^2=0.999 7), 0.116-1.160(R^2=0.999 1) had good linear relationship. The contents of sennoside A and sennoside B in Dahuang(rhubarb) with different processing methods had significant difference, illustrating different processing technologies can affect the components contents of Dahuang(rhubarb). Conclusion It established HPLC determination of sennoside A and sennoside B in different processed products of Dahuang(rhubarb) and the method can be used as methods for quality control of Dahuang(rhubarb) and its processed products.

关 键 词：大黄 炮制 番泻苷A 番泻苷B 高效液相色谱 

分 类 号：R283.1[医药卫生—中药学]