基于“肺与大肠相表里”研究清半夏多糖对哮喘模型大鼠结肠水液代谢的影响  

Effect of Pinelliae Rhizoma Praeparatum Cum Alumine Polysaccharides on Colonic Water and Liquid Metabolism in Asthma Model Rats Based on "Exterior-Interior Relationship Between Lung and Large Intestine"

作　　者：黄聪[1] 彭伟[1] 魏蜀君 牟茂婷 魏大能[1] 高永翔[1] 吴纯洁[1] HUANG Cong;PENG Wei;WEI Shujun;MOU Maoting;WEI Daneng;GAO Yongxiang;WU Chunjie(Chengdu University of Traditional Chinese Medicine,Chengdu 611137,Sichuan,China)

机构地区：[1]成都中医药大学,四川成都611137

出　　处：《中华中医药学刊》2020年第11期104-108,I0020,I0021,共7页Chinese Archives of Traditional Chinese Medicine

基　　金：国家自然科学基金面上项目(81573610)。

摘　　要：目的观察清半夏多糖对哮喘模型大鼠结肠水通道蛋白5(Aquaporin 5,AQP5)mRNA表达的影响,探讨清半夏多糖作为"大分子"多糖类成分,基于"肺与大肠相表里",对哮喘大鼠结肠水液代谢调节的作用机制。方法 48只SPF级Wistar大鼠,随机分至正常对照组、哮喘模型组、清半夏多糖高、中、低剂量组。采用鸡卵清蛋白(OVA)诱导致敏、激发,建立哮喘大鼠模型。PowerLab数据采集分析系统收集各组大鼠血氧饱和度,记录造模前后体质量变化,酶联免疫吸附测定法(ELISA)检测大鼠血清、肠黏液中核因子κB(NF-κB)的含量;苏木素-伊红(HE)、高碘酸-雪夫(PAS)染色观察结肠组织病理改变和杯状细胞数量,实时荧光定量聚合酶链式反应(qPCR)检测结肠AQP5 mRNA变化。结果哮喘模型组大鼠的血氧饱和度显著降低(P<0.01);阳性药组和清半夏多糖中剂量组大鼠血氧饱和度均有显著升高(P<0.01);哮喘模型组大鼠体质量变化不大,而正常对照组和阳性药组体质量持续显著上升(P<0.01),清半夏多糖各组大鼠体质量有上升趋势。ELISA结果显示,哮喘模型组大鼠血清和肠黏液NF-κB含量显著升高(P<0.01);而阳性药组、清半夏多糖高剂量组能显著降低哮喘模型大鼠血清、肠黏液的NF-κB的含量(P<0.01,P<0.05),清半夏多糖中剂量能显著降低哮喘大鼠肠黏液中NF-κB的含量(P<0.01)。HE染色结果发现,与正常对照组比较,哮喘模型组大鼠结肠组织出现明显病理改变,阳性药组、清半夏多糖各组与哮喘模型组比较具有缓解作用;PAS染色结果发现与哮喘模型组比较,阳性药组、清半夏多糖各组结肠杯状数量明显增多、染色加深。qPCR结果显示,阳性药组,清半夏多糖各组与哮喘模型组比较,AQP5 mRNA均有明显降低(P<0.01)。结论基于"肺与大肠相表里"理论,研究发现清半夏多糖可能通过对哮喘模型大鼠结肠AQP5 mRNA的表达从而影响结肠黏液生成,进而干预�Objective To observe the effect of Pinelliae Rhizoma Praeparatum Cum Alumine polysaccharides(PRPCAP) on the expression of aquaporin 5(AQP5) mRNA in asthmatic rats and explore the mechanism of action on the regulation of colonic water metabolism in asthmatic rats,taking PRPCAP as a "macro molecule" based on "exterior-interior relationship between lung and large intestine". Methods Forty-eight SPF-level Wistar rats were randomly assigned to the normal control group, the asthma model group, and the high, medium and low doses groups of PRPCAP. Chicken ovalbumin(OVA) was used to induce sensitization and stimulation, and the rat model of asthma was established. The PowerLab data acquisition and analysis system collected the oxygen saturation of each group. We recorded the changes of rats body weight before and after modeling. We detected the content of nuclear factor-κB(NF-κB) in serum and intestinal mucus by enzyme-linked immunosorbent assay(ELISA). Hematoxylin-Eosin(HE) and periodic acid-Schiff(PAS) staining were used to observe the pathological changes of colon tissue and the number of goblet cells. The real-time fluorescence quantitative polymerase chain reaction(qPCR) was used to detect the colonic AQP5 mRNA changes. Results The oxygen saturation of the rats in the asthma model group was significantly decreased(P<0.01). The oxygen saturation of the rats in the positive drug group and the middle dose group was significantly increased(P<0.01). The body weight of the rats in the asthma model group did not change much, but the weight of the normal control group and positive drug group continued to increase significantly(P<0.01). The results of ELISA showed that the serum and intestinal mucus NF-κB levels in the asthma model group were significantly increased(P<0.01). The positive drug group and the high-dose PRPCAP group significantly reduced the NF-κB levels in serum and intestinal mucus than asthma model(P<0.01, P<0.05). The middle dose of PRPCAP can significantly reduce the content of NF-κB in intestinal mucus

关 键 词：清半夏多糖 哮喘模型大鼠 结肠 AQP5 mRNA 

分 类 号：R285.5[医药卫生—中药学]