复配絮凝剂对圣愈汤合剂澄清效果研究  

Study on Clarification Effect of Compound Flocculant on Shengyu Decoction

作　　者：董丹华 刘玉军 孙平 李亚男 胡祥昊 高鹏[1] DONG Danhua;LIU Yujun;SUN Ping;LI Yanan;HU Xianghao;GAO Peng(School of Pharmacy,Shandong University of Traditional Chinese Medicine,Jinan 250355,Shandong,China;Shandong Yuze Yaokang Industrial Technology Research Institute Co.,Ltd.,Dezhou 251200,Shandong,China)

机构地区：[1]山东中医药大学药学院,山东济南250355 [2]山东禹泽药康产业技术研究院有限公司,山东德州251200

出　　处：《中华中医药学刊》2020年第11期121-125,I0022,I0023,共7页Chinese Archives of Traditional Chinese Medicine

基　　金：国家科技重大专项(2018ZX09721-004)。

摘　　要：目的为了提高圣愈汤合剂的澄清度,采用复配絮凝技术对圣愈汤合剂进行除杂研究。方法采用壳聚糖与明胶两种絮凝剂复配的方式对圣愈汤合剂进行除杂,采用单因素试验,以阿魏酸和毛蕊花糖苷保留率、鞣质去除率及处理后合剂浊度为指标,考察壳聚糖与明胶的配比、复配絮凝剂的用量、浓缩液含生药量、反应pH和反应温度对澄清效果的影响;采用响应面试验确定最佳絮凝工艺条件并验证。结果单因素试验确定了絮凝剂配比和用量:1%明胶与1%壳聚糖的配比为1∶2,每50 mL浓缩液复配絮凝剂的用量为3 mL;响应面试验确定了最佳絮凝工艺条件:浓缩液含生药量为0.20 g/mL,反应pH为7,反应温度为30℃。结论实验结果表明建立的絮凝方法能够选择性地去除圣愈汤合剂中的鞣质,提高合剂的澄清度,同时较好地保留了有效成分。Objective In order to improve the clarity of Shengyu Decoction, the complex flocculation technology was used to carry out the impurity removal research on Shengyu Decoction. Methods We purified Shengyu Decoction by using the combination of chitosan and gelatin. Using the single factor test, the retention ratio of ferulic acid and verbascoside, the removal rate of tannin and the turbidity of the treated mixture were used as indicators to investigate the ratio of chitosan to gelatin, the amount of flocculant used, and the amount of concentrate contained in concentrate, the reaction pH and the effect of reaction temperature on the flocculation effect. The response surface method was used to determine the optimal flocculation process and verify. Results The single factor test determined the flocculant ratio and dosage. The ratio of 1% gelatin to 1% chitosan was 1∶2, and the amount of flocculant used in each 50 mL concentrate was 3 mL. The response surface test determined the optimal flocculation process conditions. The mixture contained 0.20 g/mL of crude drug, the reaction pH was 7, and the reaction temperature was 30 ℃. Conclusion The experimental results show that the established flocculation method can selectively remove tannin in Shengyu Decoction, improve the clarity of the mixture and retain the active ingredients to a great extent.

关 键 词：圣愈汤 复配絮凝剂 响应面 

分 类 号：R284.1[医药卫生—中药学]