基于传统煎液四物汤构建的HPLC特征图谱方法的四物颗粒和四物合剂评价研究  

Evaluation of Siwu Granules and Siwu Mixtures Based on HPLC Specific Chromatogram Constructed by Traditional Siwu Decoction

作　　者：李亚男 刘玉军 董丹华 胡祥昊 孙志强 赵赞赞 代龙[1] 高鹏[1] LI Yanan;LIU Yujun;DONG Danhua;HU Xianghao;SUN Zhiqiang;ZHAO Zanzan;DAI Long;GAO Peng(School of Pharmacy,Shandong University of Traditional Chinese Medicine.Jinan 250355,Shandong,China;Qihe Peoples Hospital,Dezhou 251200,Shandong,China;Shandong Yuze Yaokang ludustricil Technology Research Institute Co.,Lld.,Dezhou 251200,Shandong,China)

机构地区：[1]山东中医药大学药学院,山东济南250355 [2]齐河县人民医院中药房,山东德州251200 [3]山东禹泽药康产业技术研究院有限公司,山东德州251200

出　　处：《中华中医药学刊》2020年第11期132-134,I0023,I0024,共5页Chinese Archives of Traditional Chinese Medicine

基　　金：国家科技重大专项(重大新药创制)(2018ZX09721-004)。

摘　　要：目的建立四物汤HPLC特征图谱的分析方法,并应用于传统煎液与成方制剂四物颗粒及四物合剂的对比,为全面评价市售四物颗粒和四物合剂质量提供依据。方法采用Inertsil ODS-SP(250 mm×4.6 mm,5μm)色谱柱,流动相为乙腈-0.1%磷酸溶液(梯度洗脱),检测波长225 nm,柱温30℃,流速为1 mL/min,运用"中药色谱指纹图谱相似度评价系统"软件对色谱图进行处理和相似度评价。结果各批次四物汤相似度均在0.97以上,标定了11个特征峰,对其中7个特征峰进行了指认。成方制剂四物颗粒与四物合剂与传统煎液对照特征谱图对比,相似度良好,分别为0.993和0.987。结论所建立的四物汤HPLC特征图谱方法,精密度、重复性和稳定性良好,能用来对四物颗粒和四物合剂进行全面的质量评价。Objective The method of HPLC specific chromatogram of Siwu Decoction was established and it was applied to the comparison between traditional decoction and Siwu Granule and Siwu Mixture, which provided a basis for comprehensive evaluation of the quality of Siwu Granules and Siwu Mixture. Method We used Inertsil ODS-SP column(250 mm×4.6 mm, 5 μm). The mobile phase was acetonitrile-0.1% phosphoric acid solution(gradient elution), the detection wavelength was 225 nm, the column temperature was 30 ℃ and the flow rate was 1 mL/min. By using Chinese medicine chromatography similarity evaluation system software,we processed and evaluated the chromatogram. Results The similarity between the prescriptions of Siwu Granules and Siwu Mixture and the traditional decoction was good, with similarities of 0.993 and 0.987, respectively. Conclusion The established HPLC specific chromatogram method of Siwu Decoction has good precision, repeatability and stability, and can be used for comprehensive quality evaluation of Siwu Granule and Siwu Mixture.

关 键 词：四物汤 四物颗粒 四物合剂 特征图谱 

分 类 号：R284.1[医药卫生—中药学]