杜仲提取物与运动联合应用对小鼠动脉粥样硬化的影响  

Effect of Eucommia Ulmoides Extract and exercise(swimming)on Atherosclerosis in Mice

作　　者：庞军 吴强[1] 李玲[1] 刘承铭 刘会[1] 李隽[1] 黄晶[1] PANG Jun;WU Qiang;LI Ling;LIU Chengming;LIU Hui;LI Yan;HUANG Jing(Department of Cardiology,Guizhou Provincial People′s Hospital,Guiyang,Guizhou 520000,China;Department of Emergency,Guizhou Provincial People′s Hospital,Guiyang,Guizhou 520000,China)

机构地区：[1]贵州省人民医院心内科,贵阳520000 [2]贵州省人民医院急诊科,贵阳520000

出　　处：《重庆医学》2020年第S02期1-3,共3页Chongqing medicine

基　　金：国家自然科学基金地区基金资助项目(81760071);国家临床重点专科建设项目(国卫办医函[2013]544号);贵州省科学技术厅临床研究中心项目[黔科合平台人才(2017)5405号];贵州省基础研究计划项目(黔科合基础[2019]1190号);贵州省中医药管理局中医药、民族医药科学技术研究课题(QZYY-2016-076);贵州省卫生健康委员会科学技术基金资助项目[gzwjkj2018-1-025]。

摘　　要：目的探究杜仲提取物与运动(游泳)联合作用对小鼠动脉粥硬化的影响。方法选取健康小鼠作为对照组,APOE-/-小鼠作为实验组,建立动脉粥样硬化模型。采用杜仲提取物进行治疗,分为高剂量组[80 mg/(kg·d)]、中剂量组[40 mg/(kg·d)]和低剂量组[20 mg/(kg·d)],并设置高脂模型组,连续给药达到疗程后通过HE染色及天狼星红染色对比各组小鼠组织染色情况,并测定小鼠NF-κB及MMP9表达情况。结果杜仲提取物结合游泳运动可明显降低小鼠胆固醇及三酰甘油水平、Ⅲ/Ⅰ型胶原比例、NF-κB及MMPS的表达,差异均有统计学意义(P<0.05)。结论杜仲提取物结合游泳运动可降低小鼠胆固醇及三酰甘油水平、Ⅲ/Ⅰ型胶原比例、NF-κB及MMPS的表达,能干预动脉粥样硬化进程。Objective In order to investigate the effect of Eucommia ulmoides extract and exercise(swimming)on atherosclerosis in mice.Methods Healthy normal mice were selected as control group,APOE-/-mice were used as experimental group to establish atherosclerosis model,and Eucommia ulmoides extract was extracted by Eucommia ulmoides The high-dose group[80 mg/(kg·d)],the middle-dose group[40 mg/(kg·d)]and the low-dose group[20 mg/(kg·d)]were treated,and a high-fat model group was set.After continuous administration reached the treatment course,The tissue staining of mice was compared by HE staining and Sirius red staining,and the expression of NF-κB and MMP9 in mice was determined.Results That Eucommia ulmoides extracts and exercise(swimming)could reduce cholesterol and triglycerides in mice(P<0.05),reduce the ratio of typeⅢ/Ⅰcollagen(P<0.05),reduce NF-κB,and MMPS expression(P<0.05).Conclusion Eucommia ulmoides extract and exercise(swimming)can reduce cholesterol and triglycerides,reduce typeⅢ/Ⅰcollagen ratio,decrease NF-κB,MMPS expression,and interfere with atherosclerosis process.

关 键 词：杜仲提纯物 APOE-/-小鼠 运动 动脉粥样硬化 

分 类 号：R28[医药卫生—中药学]